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Abstract：
Dynamic alteration of the synaptic strength is one of the most important processes occurring in the nervous system. Combination of electrophysiology, confocal imaging and molecular biology led to significant advances in this research field. Yet, a progress in this area, in particular in studies of changes in the quantal behavior of central synapses and impact of glial cells on individual synapses, is hampered by technical difficulties of resolving small quantal synaptic currents. In this paper we will show how the technique of non-enzymatic vibro-dissociation, which enables to isolate living neurons avoiding artifacts of cell culture and preserving functional synapse, can be used to obtain a valuable information on fine details and mechanisms of synaptic plasticity. In particular, we will describe our recent results on Ca2+-dependent modulation of the postsynaptic AMPA and NMDA receptors in the individual synaptic boutons.
摘要：
突触强度的动态变化是神经系统中最重要的过程之一。电生理学的组合，共聚焦成像和分子生物学导致了这一研究领域的重大进展。然而，这方面的进展，特别是在研究中枢突触的定量行为变化和神经胶质细胞对个体突触的影响时，由于解决小的量子突触电流的技术困难而受到阻碍。在本文中，我们将展示非酶振动解离技术，它能够分离活的神经元，避免细胞培养和保留功能突触的伪影，可用于获得有关突触可塑性的细节和机制的有价值的信息。特别是,我们将描述我们最近在单个突触boutons中突触后AMPA和NMDA受体的Ca2依赖性调节的结果。
