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Abstract：
Melanocyte stem cells are a population of immature cells which sustain the self-renewal and replenish the differentiated melanocytes. In this research, a light-colored region (LCR) is observed at the heel of caudal fin in juvenile crucian carp. By cutting off the caudal fin, the operated caudal fin can regenerate in accordance with the original pigment pattern from the retained LCR. As markers of stem cells, Oct4 and Sox2 have been found to be highly expressed in the LCR as well as Mitfa, a label of the melanoblasts. In vitro, Mitfa+ melanoblasts are observed in the cells which are derived from the LCR and transfected with Mitfa-EGFP reporter by using Tol2 transposon system. Furthermore, by real-time qPCR, it is shown that the level of sox2 mRNA is gradually decreased from the LCR to proximal and distal caudal fin, and that of mitfa mRNA in the proximal caudal fin (PCF) is higher than that in the LCR, while it is the lowest in the distal caudal fin. Hence, we propose that the LCR is a pigment progenitor niche, sending melanocytes to the distal of caudal fin, which gradually emerges as caudal fin grow. We reveal that the LCR of caudal fin might be a niche of pigment progenitors, and contribute to pigment-producing stem cells in crucian carp.
摘要：
黑素细胞干细胞是维持自我更新并补充分化的黑素细胞的未成熟细胞群体。在这项研究中,在幼鱼的尾鳍脚跟处观察到浅色区域（LCR）。通过切断尾鳍，操作的尾鳍可以根据保留的LCR的原始颜料图案再生。作为干细胞的标记,已发现Oct4和Sox2在LCR以及Mitfa中高度表达,黑素细胞的标签。体外，在源自LCR并通过使用Tol2转座子系统用Mitfa-EGFP报道分子转染的细胞中观察到Mitfa+黑素细胞。此外，通过实时qPCR，结果表明，从LCR到近端和远端尾鳍，sox2mRNA的水平逐渐降低，近端尾鳍（PCF）的mitfamRNA高于LCR，而它在远端尾鳍中最低。因此，我们认为LCR是一个色素祖细胞利基，将黑素细胞送到尾鳍远端,随着尾鳍的生长而逐渐出现。我们发现尾鳍的LCR可能是色素祖细胞的小生境，并有助于cru鱼产生色素的干细胞。
