Abstract：
Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult-to-find, endangered and giant species like Jaguar (Panthera onca), the largest South American felid. This unit focuses on the isolation and culture of fibroblasts from Jaguar skin and muscle biopsies, and ear cartilage dissection immediately after death to preserve one of the several endangered species in this biome. These culture techniques enabled us to contribute 570 samples from 45 autochthonous and endangered species, including Jaguar. The fibroblasts obtained are a part of the Genetic Bank of Buenos Aires Zoo with the 6700 samples, including tissues such as muscle, ovarian, testicular, blood, fibroblast cultures, sperm, hair, and fluids and cells from 450 individuals of 87 different species. © 2016 by John Wiley & Sons, Inc.
摘要：
体细胞系的建立是保存稀有遗传物质的宝贵资源,很难找到,濒危和巨型物种，如美洲虎（Pantheraonca），南美最大的猫。本单元的重点是从捷豹皮肤和肌肉活检成纤维细胞的分离和培养，死后立即进行耳软骨解剖，以保留该生物群落中几种濒临灭绝的物种之一。这些培养技术使我们能够提供来自45个本土和濒危物种的570个样本，包括捷豹.获得的成纤维细胞是布宜诺斯艾利斯动物园遗传银行的一部分，有6700个样本，包括肌肉等组织，卵巢,睾丸,血,成纤维细胞培养物,精子,头发,来自87个不同物种的450个个体的液体和细胞。©2016由JohnWiley&Sons，Inc.
