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Abstract：
Using LAURDAN spectral imaging and spectral phasor analysis we concurrently studied the growth and hydration state of subcellular organelles (lamellar body-like, LB-like) from live A549 lung cancer cells at different post-confluence days. Our results reveal a time dependent two-step process governing the size and hydration of these intracellular LB-like structures. Specifically, a first step (days 1 to 7) is characterized by an increase in their size, followed by a second one (days 7 to 14) where the organelles display a decrease in their global hydration properties. Interestingly, our results also show that their hydration properties significantly differ from those observed in well-characterized artificial lamellar model membranes, challenging the notion that a pure lamellar membrane organization is present in these organelles at intracellular conditions. Finally, these LB-like structures show a significant increase in their hydration state upon secretion, suggesting a relevant role of entropy during this process.
摘要：
使用LAURDAN光谱成像和光谱相量分析，我们同时研究了亚细胞器的生长和水合状态（层状体状，LB样）来自不同汇合后天数的活A549肺癌细胞。我们的结果表明，时间依赖性的两步过程控制这些细胞内LB样结构的大小和水合。具体来说,第一步(第1天至第7天)的特征是它们的尺寸增加，其次是第二个（第7至14天），其中细胞器显示其整体水合性能下降。有趣的是,我们的结果还表明，它们的水化特性与在特征良好的人工层状模型膜中观察到的明显不同，挑战在细胞内条件下这些细胞器中存在纯层状膜组织的概念。最后,这些LB样结构在分泌时显示其水合状态显着增加，表明熵在这一过程中的相关作用。
