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Abstract：
We performed a genome-wide association study (GWAS) and candidate gene analysis to: (i) evaluate the effectiveness of the GWAS in our small population by performing GWAS for carcass weight (CW) and fatty acid composition; (ii) detect novel candidate regions affecting non-CW carcass traits, chemical composition and sugar; and (iii) evaluate the association of the candidate genes previously detected in CW and fatty acid composition with other economically important traits. A total of 574 Japanese Black cattle and 40 657 Single nucleotide polymorphisms were used. In addition, candidate gene analyses were performed to evaluate the association of three CW-related genes and two fatty acid-related genes with carcass traits, fatty acid composition, chemical composition and sugar. The significant regions with the candidate genes were detected for CW and fatty acid composition, and these results showed that a significant region would be detectable despite the small sample size. The novel candidate regions were detected on BTA23 for crude protein and on BTA19 for fructose. CW-related genes associated with the rib-eye area and fatty acid composition were identified, and fatty acid-related genes had no relationship with other traits. Moreover, the favorable allele of CW-related genes had an unfavorable effect on fatty acid composition.
摘要：
我们进行了全基因组关联研究（GWAS）和候选基因分析，以：（i）通过对car体重量（CW）和脂肪酸组成进行GWAS来评估GWAS在我们小人群中的有效性；（ii）检测影响非CWcar体性状的新候选区域，化学成分和糖；（iii）评估先前在CW和脂肪酸组成中检测到的候选基因与其他经济上重要的性状的关联。总共使用了574只日本黑牛和40657个单核苷酸多态性。此外,进行了候选基因分析，以评估三个CW相关基因和两个脂肪酸相关基因与car体性状的关联，脂肪酸组成，化学成分和糖。检测到具有候选基因的重要区域的CW和脂肪酸组成，这些结果表明，一个显著的区域将是可检测的，尽管小样本量。在BTA23上检测到粗蛋白和在BTA19上检测到果糖的新候选区域。确定了与肋眼面积和脂肪酸组成相关的CW相关基因，脂肪酸相关基因与其他性状无关。此外，CW相关基因的有利等位基因对脂肪酸组成有不利影响。
