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Abstract：
Here we report occurrence of six different morphotypes of ascaridoid type larvae from 28 species of fish collected from New Caledonian waters. The larvae were morphologically identified as Anisakis type I, Hysterothylacium type VI and new larval types XIII and XIV, Raphidascaris larval type and Terranova larval type II. Representatives of each morphotype were subjected to the amplification of the second internal transcribed spacers (ITS-2) of ribosomal DNA (rDNA) and those sequences were compared with ITS-2 sequences of other ascaridoid nematodes previously deposited in GenBank. ITS-2 sequences of Anisakis larval type I were identical to those of A. typica. ITS-2 sequences of Hysterothylacium larval type VI in the present study were identical to those previously found in Eastern Australian waters. No match was found for ITS-2 sequences of Hysterothylacium larval types XIII and XIV; therefore, the specific identities of these larval types remain unclear. ITS-2 sequences of Raphidascaris larval type were identical to those of R. trichiuri, which have previously been reported in Taiwanese waters. Terranova larval type II in the present study had identical ITS-2 sequences with Terranova larval types reported from Australian waters, however, the specific identity is unknown. This taxonomic work is essential if further research on these zoonotic parasites is to be effective. This includes investigations into such aspects as life cycle studies, impacts on human health and risk assessment for their transmission to humans.
摘要：
在这里，我们报告了从新喀里多尼亚水域收集的28种鱼中6种不同形态的a虫类型幼虫的发生。幼虫在形态上被鉴定为I型Anisakis，六型和新的幼虫类型XIII和XIV，Rephidasc幼虫型和Terranova幼虫II型。对每种形态类型的代表进行核糖体DNA（rDNA）的第二个内部转录间隔区（ITS-2）的扩增，并将这些序列与先前存放在GenBank中的其他类as虫线虫的ITS-2序列进行比较。I型Anisakis幼虫的ITS-2序列与典型A.的ITS-2序列相同。本研究中的VI型Hysterothylacium幼虫的ITS-2序列与先前在澳大利亚东部水域发现的序列相同。未发现与之匹配的Hysterothylacium幼虫类型XIII和XIV的ITS-2序列；因此，这些幼虫类型的具体身份仍不清楚。Raphidasc幼虫类型的ITS-2序列与Trichiuri的相同，此前曾在台湾水域报道过。本研究中的TerranovaII型幼虫具有与澳大利亚水域报道的Terranova幼虫类型相同的ITS-2序列，然而,具体身份不明。如果要对这些人畜共患寄生虫进行进一步研究，这项分类工作至关重要。这包括对生命周期研究等方面的调查，对人类健康的影响及其传播给人类的风险评估。
