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Abstract：
A preliminary linkage map was constructed by applying backcross and testcross strategy using microsatellite (SSR) markers developed for Xiphophorus and Poecilia reticulata in ornamental fish, molly Poecilia sp. The linkage map having 18 SSR loci consisted of four linkage groups that spanned a map size of 516.1cM. Association between genotypes and phenotypes was tested in a random fashion and QTL for dorsal fin length was found to be linked to locus Msb069 on linkage group 2. Coincidentally, locus Msb069 was also reported as putative homologue primer pairs containing SSRs repeat motif which encoded hSMP-1, a sex determining locus. Dorsal fin length particularly in males of Poecilia latipinna is an important feature during courtship display. Therefore, we speculate that both dorsal fin length and putative hSMP-1 gene formed a close proximity to male sexual characteristics.
摘要：
通过使用为观赏鱼中的Xiphophorus和Poeciliareticulata开发的微卫星（SSR）标记应用回交和testcross策略，构建了初步的连锁图。MollyPoeciliasp.具有18个SSR基因座的连锁图谱由四个连锁群组成，其图谱大小为516.1cM。以随机方式测试了基因型和表型之间的关联，发现背鳍长度的QTL与连锁群2上的基因座Msb069连锁。巧合的是,基因座Msb069也被报道为推定的同源引物对，其中包含SSR重复基序，该基序编码hSMP-1，这是一个性别决定基因座。背鳍长度，特别是在Poecilialatipinna的雄性中，是求偶显示过程中的重要特征。因此,我们推测背鳍长度和推定的hSMP-1基因与男性性特征非常接近。
