通过慢病毒基因疗法抑制产前缺乏 CLN5 的绵羊神经培养物中的储存病理学。
关键词: 4′,6-diamidino-2-phenylindole AAV Batten disease CIDR CLN5 DAPI Endo H Endoglycosidase H FBS Fluorescent storage bodies GFAP GFP Gene therapy LVMNDU3 Lentiviral vector M6P M6PR MAP MOI NCL Neural cell culture Neuronal ceroid lipofuscinoses PAGE PBS PDI PNGase F PVDF SDS Sheep TU Transduction adeno-associated virus controlled internal drug (progesterone) release device days in vitro div foetal bovine serum glial fibrillary acidic protein green fluorescent protein lentiviral derived vector with a myeloid sarcoma virus U3 element mannose-6-phosphate mannose-6-phosphate receptor microtubule associated protein multiplicity of infection neuronal ceroid lipofuscinosis peptide-N-glycosidase F phosphate buffered saline, pH7.2 polyacrylamide gel electrophoresis polyvinylidene fluoride protein disulphide isomerase sodium dodecyl sulphate transducing units
Mesh : Amino Acid Sequence Animals Genetic Therapy HEK293 Cells Humans Lentivirus / genetics Lysosomal Membrane Proteins Membrane Proteins / genetics metabolism Molecular Sequence Data Neuronal Ceroid-Lipofuscinoses / embryology genetics metabolism pathology Neurons / metabolism pathology Sheep
来 源:
DOI:10.1016/j.nbd.2013.11.011
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