The U6 gene of Saccharomyces cerevisiae is transcribed by RNA polymerase C (III) in vivo and in vitro.

酿酒酵母的 U6 基因在体内和体外被 RNA 聚合酶 C (III) 转录。

• 影响指数: 14.012
• 发表时间: Jan 1990
• 来源期刊: EMBO J
• DOI: 10.1002/j.1460-2075.1990.tb08105.x
• PMID: 2403927
• 文章类型: Journal Article
• 中科院分区: Q1
• 方向: 生物
• 作者列表: Moenne A,Camier S,Anderson G,Margottin F,Beggs J,Sentenac A

Mesh: Base Sequence; DNA-Directed RNA Polymerases / metabolism; Molecular Sequence Data; Mutation; Nucleic Acid Hybridization; RNA Polymerase III / metabolism; RNA, Ribosomal, 5S / genetics; RNA, Small Nuclear / genetics; RNA, Transfer / genetics; Restriction Mapping; Saccharomyces cerevisiae / genetics; Single-Strand Specific DNA and RNA Endonucleases; Transcription Factor TFIIIB; Transcription Factors / pharmacology; Transcription, Genetic

Abstract

Unlike the majority of genes encoding small nuclear RNAs, which are transcribed by RNA polymerase B, the U6 gene contains features found in both class B and class C genes, indicating the involvement of a combination of transcription factors normally specific to each class of genes. We present direct genetic and biochemical evidence that the U6 gene of Saccharomyces cerevisiae is transcribed by RNA polymerase C in vivo as well as in vitro. A mutant strain with a temperature-sensitive defect in the large subunit of RNA polymerase C that results in defective transcription of tRNA and 5S RNA genes shows a corresponding defect in U6 RNA levels. Also, purified RNA polymerase C transcribes the U6 gene when supplemented with partially purified TFIIIB. The other class C transcription factors, TFIIIA and Tau (TFIIIC), are not required in this system.