U4 和 U6 RNA 共存于单个小核核糖核蛋白颗粒中。U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle.-医云文献数字医云科研云海量医学决策数据服务

Abstract：

U4 and U6 RNAs of mammalian cells possess extensive intermolecular sequence complementarity and hence have the potential to base pair. A U4/U6 RNA complex, detectable in nondenaturing polyacrylamide gels, is released when human small nuclear ribonucleoproteins (snRNPs) containing U1, U2, U4, U5, and U6 RNAs are dissociated with proteinase K in the presence of sodium dodecyl sulfate. The released RNA/RNA complex dissociates with increasing temperature, consistent with the existence of specific base-pairing between the two RNAs. Since U6 RNA is selectively released from intact snRNPs under the same conditions required to dissociate the U4/U6 RNA complex, the RNA-RNA interaction may be sufficient to maintain U4 and U6 RNAs in the same snRNP particle. The biological implications of these findings are discussed.

摘要：

哺乳动物细胞的U4和U6RNA具有广泛的分子间序列互补性，因此具有碱基配对的潜力。U4/U6RNA复合物，在非变性聚丙烯酰胺凝胶中检测到，当含有U1，U2，U4，U5和U6RNA的人小核核糖核蛋白（snRNP）在十二烷基硫酸钠存在下用蛋白酶K解离时释放。释放的RNA/RNA复合物随着温度升高而解离，与两种RNA之间存在特定的碱基配对一致。由于U6RNA在解离U4/U6RNA复合物所需的相同条件下从完整的snRNP中选择性释放，RNA-RNA相互作用可能足以将U4和U6RNA维持在同一snRNP颗粒中。讨论了这些发现的生物学意义。

U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle.

1 Studies on transfer ribonucleic acids and related compounds. XXXII. Synthesis of ribonucleotides corresponding to residues 1-5 and 6-10 of tRNAfMet from E. coli and their base conversion analogs.

2 Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus.

3 5.8S ribosomal RNA.

4 Fractionation and characterization of human small nuclear ribonucleoproteins containing U1 and U2 RNAs.

5 Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.

6 Primary and secondary structures of chicken, rat and man nuclear U4 RNAs. Homologies with U1 and U5 RNAs.

7 Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.

8 Primary and secondary structure of U2 snRNA.

9 Ribosomal RNA evolution by fragmentation of the 23S progenitor: maturation pathway parallels evolutionary emergence.

10 The nucleotide sequence of nuclear 4.8S RNA of mouse cells.

11 A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.

12 Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.

13 The nuclear 5S RNAs from chicken, rat and man. U5 RNAs are encoded by multiple genes.

14 Nucleotide sequences of nuclear U1A RNAs from chicken, rat and man.

15 Molecular characterization of ribonucleoprotein antigens bound by antinuclear antibodies. A diagnostic evaluation.

16 Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera.

17 How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.

18 Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.

19 SnRNAs, SnRNPs, and RNA processing.

20 Structural organization of ribonucleoproteins containing small nuclear RNAs from HeLa cells. Proteins interact closely with a similar structural domain of U1, U2, U4 and U5 small nuclear RNAs.

21 5'-terminal caps of snRNAs are accessible for reaction with 2,2,7-trimethylguanosine-specific antibody in intact snRNPs.

22 Sequence determination of rat U5 RNA using a chemical modification procedure for counteracting sequence compression.

23 Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.

24 U2 RNA shares a structural domain with U1, U4, and U5 RNAs.

25 Human U1 and U2 small nuclear ribonucleoproteins contain common and unique polypeptides.

26 A U4-like small nuclear RNA is dispensable in yeast.

27 Are snRNPs involved in splicing?

28 Sequencing end-labeled DNA with base-specific chemical cleavages.

29 The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro.

30 Isolation of small nuclear ribonucleoproteins containing U1, U2, U4, U5, and U6 RNAs.

31 The nucleotide sequence of nuclear U6 (4.7 S) RNA.