PDF(Pubmed)
Abstract：
Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as α-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-40℃) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae.
摘要：
已知单纯性Anisakis的幼虫排泄分泌产物会引起人类的过敏反应。用针对A.simplexL3排泄分泌产物的多克隆兔血清免疫筛选了A.simplex3期幼虫（L3）的cDNA文库，以鉴定引发免疫反应的抗原。一个cDNA克隆，命名为α-甲基酰基CoA消旋酶（Amacr），包含1,412bp的cDNA转录本，具有编码418个氨基酸的单个开放阅读框。与其他物种的Amacr直向同源物相比，A.simplexAmacr显示出高度的同源性。与温度（10-40℃）和时间（24-48小时）无关，AmacrmRNA高度且组成型表达。免疫组织化学分析显示，Amacr主要在单纯幼虫的脑室中表达。从脑室大量产生的Amacr蛋白可能在单纯幼虫的发育和生长中起许多作用。
