PDF(Pubmed)
Abstract：
Previous studies have demonstrated that macromolecular synthesis in the brain is modulated in association with the occurrence of sleep and wakefulness. Similarly, the spectral composition of electroencephalographic activity that occurs during sleep is dependent on the duration of prior wakefulness. Since this homeostatic relationship between wake and sleep is highly conserved across mammalian species, genes that are truly involved in the electroencephalographic response to sleep deprivation might be expected to be conserved across mammalian species. Therefore, in the rat cerebral cortex, we have studied the effects of sleep deprivation on the expression of immediate early gene and heat shock protein mRNAs previously shown to be upregulated in the mouse brain in sleep deprivation and in recovery sleep after sleep deprivation. We find that the molecular response to sleep deprivation and recovery sleep in the brain is highly conserved between these two mammalian species, at least in terms of expression of immediate early gene and heat shock protein family members. Using Affymetrix Neurobiology U34 GeneChips , we also screened the rat cerebral cortex, basal forebrain, and hypothalamus for other genes whose expression may be modulated by sleep deprivation or recovery sleep. We find that the response of the basal forebrain to sleep deprivation is more similar to that of the cerebral cortex than to the hypothalamus. Together, these results suggest that sleep-dependent changes in gene expression in the cerebral cortex are similar across rodent species and therefore may underlie sleep history-dependent changes in sleep electroencephalographic activity.
摘要：
先前的研究表明，大脑中的大分子合成与睡眠和觉醒的发生有关。同样，睡眠期间脑电图活动的频谱组成取决于先前觉醒的持续时间。由于清醒和睡眠之间的平衡关系在哺乳动物物种中高度保守，真正参与脑电图对睡眠剥夺反应的基因有望在哺乳动物物种中得到保留。因此,在大鼠大脑皮层，我们研究了睡眠剥夺对即刻早期基因和热休克蛋白mRNAs表达的影响，这些基因和热休克蛋白mRNAs在睡眠剥夺和睡眠剥夺后的恢复睡眠中显示出上调。我们发现，大脑中对睡眠剥夺和恢复睡眠的分子反应在这两种哺乳动物物种之间是高度保守的，至少在即时早期基因和热休克蛋白家族成员的表达方面。使用Affymetrix神经生物学U34基因芯片，我们还筛查了大鼠大脑皮层，基底前脑，和下丘脑的其他基因，其表达可能受到睡眠剥夺或恢复睡眠的调节。我们发现，基底前脑对睡眠剥夺的反应更类似于大脑皮层，而不是下丘脑。一起,这些结果表明,睡眠依赖性大脑皮层基因表达的变化在不同啮齿动物物种中是相似的,因此可能是睡眠史依赖性睡眠脑电图活动变化的基础.
