Abstract：
OBJECTIVE: To investigate the effects of FGF-8 on cranial neural crest cell (CNCC) differentiating into ectomesenchymal cell of the first branchial arch, and determine the appropriate dose and stage of CNCC exposure to FGF-8.
METHODS: Cranial neural crest explants were cultured in free-serum medium containing modified DMEM/F12 and different doses of FGF-8. The differentiation type of CNCC were determined by in situ hybridization for Hoxa2 and immunocytochemistry for vimentin.
RESULTS: Pre-emigrating CNCC demonstrated the negative Hoxa2 stain and positive vimentin stain after treated by 100 ug/FGF-8. Both post-emigrating CNCC group and control group were positive for Hoxa2 and vimentin stain.
CONCLUSIONS: On the early stage of CNCC emigration, the first branchial arch phenotype of CNCC could be induced by FGF-8. This experiment could provide in vitro model for study on the mechanism of tooth-jaw regeneration.
METHODS: Cranial neural crest explants were cultured in free-serum medium containing modified DMEM/F12 and different doses of FGF-8. The differentiation type of CNCC were determined by in situ hybridization for Hoxa2 and immunocytochemistry for vimentin.
RESULTS: Pre-emigrating CNCC demonstrated the negative Hoxa2 stain and positive vimentin stain after treated by 100 ug/FGF-8. Both post-emigrating CNCC group and control group were positive for Hoxa2 and vimentin stain.
CONCLUSIONS: On the early stage of CNCC emigration, the first branchial arch phenotype of CNCC could be induced by FGF-8. This experiment could provide in vitro model for study on the mechanism of tooth-jaw regeneration.
摘要：
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