%0 English Abstract
%T [In vitro study on cranial neural crest differentiating into ectomesenchymal cell of the first branchial arch by FGF-8].
%A Jiang HB
%A Tian WD
%A Tang W
%A Liu L
%A Li XD
%J Zhonghua Kou Qiang Yi Xue Za Zhi
%V 40
%N 4
%D Jul 2005
%M 16191378
暂无%X <B>OBJECTIVE: </B>To investigate the effects of FGF-8 on cranial neural crest cell (CNCC) differentiating into ectomesenchymal cell of the first branchial arch, and determine the appropriate dose and stage of CNCC exposure to FGF-8.<BR><B>METHODS: </B>Cranial neural crest explants were cultured in free-serum medium containing modified DMEM/F12 and different doses of FGF-8. The differentiation type of CNCC were determined by in situ hybridization for Hoxa2 and immunocytochemistry for vimentin.<BR><B>RESULTS: </B>Pre-emigrating CNCC demonstrated the negative Hoxa2 stain and positive vimentin stain after treated by 100 ug/FGF-8. Both post-emigrating CNCC group and control group were positive for Hoxa2 and vimentin stain.<BR><B>CONCLUSIONS: </B>On the early stage of CNCC emigration, the first branchial arch phenotype of CNCC could be induced by FGF-8. This experiment could provide in vitro model for study on the mechanism of tooth-jaw regeneration.