目的:七氟醚和丙泊酚是手术中最常用的麻醉药。在这项研究中,目的探讨七氟醚和丙泊酚在结直肠癌中的作用。
方法:细胞计数试剂盒-8,集落形成,westernblot,并进行transwell测定以确定细胞增殖,凋亡,铁性凋亡,入侵,和移民。我们进行过表达实验以检测七氟醚和异丙酚的潜在分子机制。通过蛋白质印迹测量与上皮-间质转化相关的基因。
结果:我们发现七氟醚和异丙酚联合治疗比七氟醚或异丙酚在体外治疗结直肠癌细胞中具有更多的抗肿瘤活性。机械上,我们的数据显示七氟醚和丙泊酚诱导细胞凋亡和铁凋亡并抑制细胞增殖,入侵,和移民。此外,TM2D1被认为是七氟醚和异丙酚的靶标,TM2D1的过表达可以逆转七氟醚和异丙酚对大肠癌细胞生物学行为的影响。
结论:我们的结果表明七氟醚和异丙酚在结直肠癌细胞中具有新的抗肿瘤机制,TM2D1可能是治疗结直肠癌患者的潜在治疗靶点。
OBJECTIVE: Sevoflurane and propofol are the most commonly used
anesthetics in surgery. In this study, we aim to explore and clarify the function of
sevoflurane and propofol in colorectal cancer.
METHODS: Cell counting kit-8, colony formation, western blot, and transwell assays were performed to determine cell proliferation, apoptosis, ferroptosis, invasion, and migration. We performed overexpression experiments to detect the underlying molecular mechanism of sevoflurane and propofol. The genes related to epithelial-mesenchymal transition were measured by western blot.
RESULTS: We discovered that
sevoflurane and propofol co-treatment exerted more anti-tumor activities than just
sevoflurane or propofol treatment in colorectal cancer cells in vitro. Mechanistically, our data showed that sevoflurane and propofol-induced apoptosis and ferroptosis and inhibited cell proliferation, invasion, and migration. Additionally, TM2D1 was considered a target of sevoflurane and propofol, and TM2D1 overexpression reversed the effect of
sevoflurane and propofol on colorectal cancer cell biology behaviors.
CONCLUSIONS: Our results showed a novel anti-tumor mechanism of sevoflurane and propofol in colorectal cancer cells, and TM2D1 might be an underlying therapeutic target for treating colorectal cancer patients.