■这项研究旨在通过PCR的显微镜和分子分析来检测猫中的弓形虫,测序,测序和系统发育分析。
■随机选择的200个猫粪便样本来自巴格达的各个私人兽医诊所。通过浮选法鉴定鱼卵,从100只猫粪便中提取DNA,一对ITS2区域特异性引物用于聚合酶链反应,其次是测序。
■使用PCR发现,在100个粪便样本中,弓形虫感染率为23个。十项DNA产物序列数据研究显示与基因库中发现的5.8S核糖体RNA基因序列具有98%-100%的相似性。研究表明,显微镜检查的总体感染率为23%,流浪猫之间没有显着差异(27%),和家猫(19%)。在研究了几个流行病学参数对感染率的影响后,发现在六个月以下的小猫中,流浪猫和家猫的感染率更高,分别为46.1%和27%,分别,而成年猫的发病率低于6个月,家猫的发病率为11.5%,流浪猫的发病率为14.7%。注册的流浪和家养雄性猫的百分比为35.5%,而注册的雌性猫分别为20.6%和17.5%,分别。
■猫是人畜共患寄生虫的重要临床储库。在伊拉克,巴格达的T.cati检测发生率很高。与传统方法相比,PCR被认为是一种更敏感的,确认物种身份的准确诊断程序。
UNASSIGNED: This study aimed to detect Toxocara cati in cats by microscopic and molecular analysis using PCR, sequencing, and phylogenetic analysis.
UNASSIGNED: Randomly selected 200 cat feces samples were taken from various private veterinarian clinics in Baghdad. To identify eggs of T. cati by the flotation method, DNA from 100 cat feces was extracted, and one pair of ITS2 region-specific primers was used for polymerase chain reaction, followed by sequencing.
UNASSIGNED: Toxocara cati infection rate was found to be 23 out of 100 fecal samples using PCR. Ten DNA product sequence data studies showed 98%-100% similarity to the 5.8S ribosomal RNA gene sequences found in the Gene Bank. The study incidence showed that the overall infection rate by microscopic examination was 23%, with no significant difference between stray cats (27%), and domestic cats (19%). After studying the effect of several epidemiological parameters on the infection rate, it was found that the infection rates of stray and domestic cats were higher in kittens under six months of age, at 46.1% and 27%, respectively, whereas rates were lower for the adult than six months was 11.5% of domestic cats and 14.7% of stray cats. The percentage of stray and domestic male cats that were registered was 35.5%, whereas the female cats registered were 20.6% and 17.5%, respectively.
UNASSIGNED: Cats are significant clinical reservoirs for zoonotic parasites. In Iraq, Baghdad has a high incidence of T. cati detections. Compared to conventional methods, PCR is thought to be a more sensitive, accurate diagnostic procedure that confirms the species\' identity.