我们研究了短期饮食锌缺乏对锌和钙代谢的影响。将四周大的雄性Wistar大鼠分为两个配对喂养组,进行1周治疗:锌缺乏组(ZD,1ppm);对照组(PF,30ppm)。锌转运蛋白的mRNA表达,如Slc39a(Zip)4、Zip5、Zip10和Slc30a(ZnT)1,在各种组织(肝脏,肾,和十二指肠)对饮食中的锌缺乏反应迅速。尽管PF组和ZD组之间的血清钙浓度没有显着差异,ZD组的血清1,25-二羟基胆钙化醇(1,25(OH)2D3)高于PF组。此外,短期缺锌显著增加瞬时受体电位(TRP)阳离子通道亚家族香草素(V)成员6、S100钙结合蛋白G(S100g)的mRNA表达,和十二指肠中ATPase质膜Ca2+转运1(Atp2b1)。此外,短期锌缺乏会增加肾脏中维生素D受体(VDR)和细胞色素P450家族24亚家族A成员1(Cyp24a1)mRNA的表达。这些发现表明,短期锌缺乏通过十二指肠中Ca吸收相关基因表达维持血清钙浓度,短期锌缺乏会响应血清1,25(OH)2D3水平的升高而诱导肾脏中Cyp24a1的表达。
We investigated the effects of short-term dietary zinc deficiency on zinc and calcium metabolism. Four-week-old male Wistar rats were divided into two pair-fed groups for a 1-wk treatment: zinc-deficient group (ZD, 1 ppm); control group (PF, 30 ppm). The mRNA expression of zinc transporters, such as Slc39a (Zip) 4, Zip5, Zip10, and Slc30a (ZnT) 1, in various tissues (liver, kidney, and duodenum) quickly responded to dietary zinc deficiency. Although there was no significant difference in serum calcium concentrations between the PF and ZD groups, serum 1,25-dihydroxycholecalciferol (1,25(OH)2D3) was higher in the ZD group than in the PF group. Moreover, short-term zinc deficiency significantly increased mRNA expression of transient receptor potential (TRP) cation channel subfamily vanilloid (V) member 6, S100 calcium binding protein G (S100g), and ATPase plasma membrane Ca2+ transporting 1 (Atp2b1) in the duodenum. Furthermore, short-term zinc deficiency increased vitamin D receptor (VDR) and cytochrome P450 family 24 subfamily A member 1 (Cyp24a1) mRNA expression in the kidney. These findings suggested that short-term zinc deficiency maintains serum calcium concentrations through Ca absorption-related gene expression in the duodenum, and that short-term zinc deficiency induced the expression of Cyp24a1 in kidney in response to an increase in the serum 1,25(OH)2D3 level.