■本研究旨在揭示从花生根瘤中分离出的乘客内生细菌阴沟肠杆菌S23,并支持负责非生物胁迫耐受性的分子机制和基因。
■除结瘤根瘤菌外,还有助于结瘤并促进植物发育活动的多种微生物。乘客内生菌(PE)是在没有任何选择压力的情况下意外穿透植物的内生菌,将它们保持在植物的内部组织中。PE具有促进植物发育和提高产量同时减少病原体感染和提高生物和非生物胁迫耐受性的特征。然而,缺乏关于乘客内生菌介导的非生物胁迫缓解的分子证据。
■制定这项研究是为了揭示阴沟肠杆菌S23的基因组序列草案,以及与植物生长促进和胁迫耐受性有关的基因和特征。
■将数据提交给PATRIC,并使用TORMES-1.0Unicycker工具对阴沟肠杆菌S23进行了完整的基因组研究。TORMES-1.0平台用于处理读取。用RAST工具包(RASTtk)注释S23序列。植物生长促进性状,如吲哚乙酸生产,铁载体分泌,胞外多糖的生产,生物膜的形成,磷酸盐溶解,并在正常情况下检查渗透物质的积累,7%NaCl和30%聚乙二醇修正条件,以确定它们承受盐和水分胁迫条件的能力,分别。
■我们报告阴沟肠杆菌S23的大小为4.82Mb,其中包含4511个蛋白质编码序列,71个转移RNA基因,3核糖体RNA中G+C一DNA含量为55.10%。功能分析显示大部分基因参与氨基酸的代谢,辅因子,维生素,应激反应,养分溶解(kdp,pho,pst),生物膜形成(pga)IAA生产(trp),铁载体生产(luc,fhu,fep,ent,ybd),防御,和毒力。结果表明,阴沟肠球菌S23在非生物胁迫条件下表现出多种植物促生长性状。
■我们的研究表明,预期基因和代谢途径的发现可能将这种细菌描述为一种环境友好的生物资源,通过多种胁迫下的几种作用机制来支持花生的生长。
UNASSIGNED: This study aims to reveal the passenger endophytic bacterium Enterobacter cloacae S23 isolated from groundnut nodules and to underpin the molecular mechanism and genes responsible for abiotic stress tolerance.
UNASSIGNED: A variety of microorganisms that contribute to nodulation and encourage plant development activity in addition to the nodulating Rhizobium. Passenger endophytes (PE) are endophytes that accidentally penetrate the plant without any selective pressure keeping them in the interior tissue of the plant. PE possesses characteristics that encourage plant development and boost output while reducing pathogen infection and improving biotic and abiotic stress tolerance. However, there is a lack of molecular evidence on the passenger endophyte-mediated alleviation of abiotic stresses.
UNASSIGNED: This study was formulated to reveal the draft genome sequence of Enterobacter cloacae S23, as well as genes and characteristics involved in plant growth promotion and stress tolerance.
UNASSIGNED: The data were submitted to PATRIC and the TORMES-1.0 Unicyclker tools were used to conduct a complete genome study of Enterobacter cloacae S23. The TORMES-1.0 platform was used to process the reads. RAST tool kit (RASTtk) was used to annotate the S23 sequence. The plant growth-promoting traits such as indole acetic acid production, siderophore secretion, production of extracellular polysaccharides, biofilm formation, phosphate solubilization, and accumulation of osmolytes were examined under normal, 7% NaCl and 30% polyethylene glycol amended conditions to determine their ability to withstand salt and moisture stressed conditions, respectively.
UNASSIGNED: We report the size of Enterobacter cloacae S23 is 4.82Mb which contains 4511 protein-coding sequences, 71 transfer RNA genes, and 3 ribosomal RNA with a G+C content of DNA is 55.10%. Functional analysis revealed that most of the genes are involved in the metabolism of amino acids, cofactors, vitamins, stress response, nutrient solubilization (kdp, pho, pst), biofilm formation (pga) IAA production (trp), siderophore production (luc, fhu, fep, ent, ybd), defense, and virulence. The result revealed that E. cloacae S23 exhibited multiple plant growth-promoting traits under abiotic stress conditions.
UNASSIGNED: Our research suggested that the discovery of anticipated genes and metabolic pathways might characterise this bacterium as an environmentally friendly bioresource to support groundnut growth through several mechanisms of action under multi-stresses.