目的:强迫症(OCD)是一种复杂的精神疾病。遗传和广泛的环境因素是强迫症的常见危险因素。目的探讨强迫症的分子机制,寻找诊断和治疗强迫症的新分子靶点。
方法:所有数据均从公共数据集下载。基于加权基因共表达网络分析(WGCNA)鉴定关键模块和候选关键mRNA。“limma”R包用于mRNA的差异表达分析。随后,还进行了差异表达的mRNAs(DEmRNAs)的功能富集分析。此外,建立了诊断模型。最后,分析OCD中免疫细胞的浸润水平及其与多中心关键DEmRNAs的相关性。
结果:选择绿色和红色模块作为集线器模块。根据GS>0.2和MM>0.3,总共447个mRNA被认为是候选关键mRNA。在GSE60190和GSE78104数据集中鉴定了相同方向的总共26个DEmRNA。共有26个DEmRNAs与WGCNA中的候选关键mRNA交叉,以获得10个交叉DEmRNAs(HSPB1,ITPK1,CBX7,PPP1R10,TAOK1,PISD,MKNK2、RWDD1、PPA1和RELN)。然而,只有4种DMRNAs(HSPB1、TAOK1、MKNK2和PPA1)预测相关药物。随后,接收机工作特性分析表明,该诊断模型具有较高的诊断价值。此外,六个多中心关键DMRNAs(SNRPF,SNRNP70,PRPF8,NOP56,EPRS,和CCT2)通过UpSet包进行筛选。最后,研究发现6种多中心关键DMRNAs与免疫细胞相关。
结论:本研究获得的关键分子为进一步研究强迫症的分子机制奠定了基础。
OBJECTIVE: Obsessive-compulsive disorder (OCD) is a complex psychiatric disorder. Genetic and broad environmental factors are common risk factors for OCD. The purpose of this study is to explore the molecular mechanism of OCD and to find new molecular targets for the diagnosis and management of OCD.
METHODS: All data were downloaded from public dataset. Key modules and candidate key mRNAs were identified based on weighted gene co-expression network analysis (WGCNA). The \"limma\" R package was used for differential expression analysis of mRNAs. Subsequently, functional enrichment analysis of differentially expressed mRNAs (DEmRNAs) was also carried out. In addition, a diagnostic model was constructed. Finally, the infiltration level of immune cells in OCD and its correlation with multicentric key DEmRNAs were analyzed.
RESULTS: Green and red modules were selected as the hub modules. A total of 447 mRNAs were considered candidate key mRNAs according to GS > 0.2 and MM > 0.3. A total of 26 DEmRNAs in the same direction were identified in the GSE60190 and GSE78104 datasets. A total of 26 DEmRNAs were intersected with candidate key mRNAs in WGCNA to obtain 10 intersection DEmRNAs (HSPB1, ITPK1, CBX7, PPP1R10, TAOK1, PISD, MKNK2, RWDD1, PPA1, and RELN). However, only four DEmRNAs (HSPB1, TAOK1, MKNK2, and PPA1) predicted related drugs. Subsequently, receiver operating characteristic analysis shows that the diagnostic model has high diagnostic value. Moreover, six multicentric key DEmRNAs (SNRPF, SNRNP70, PRPF8, NOP56, EPRS, and CCT2) were screened by UpSet package. Finally, six multicentric key DEmRNAs were found to be associated with immune cells.
CONCLUSIONS: The key molecules obtained in this study lay a foundation for further research on the molecular mechanism of OCD.