■西瓜是葫芦科的一年生藤本植物。西瓜植物产生人们喜爱的水果,具有重要的营养和经济价值。随着全球气候变暖和生态环境的恶化,非生物胁迫,包括干旱,成为影响西瓜产量和品质的重要因素。先前关于西瓜抗旱性的研究包括基于其他物种中已知的干旱响应基因和途径分析同源基因。
■然而,通过高通量组学方法鉴定与西瓜抗旱相关的关键通路和基因尤为重要。在这项研究中,在五个时间点(0h,1h,6h,12h和24h)干旱胁迫前后。
■转录组学分析揭示了在五个时间点的7829个差异表达基因(DEGs)。使用k均值聚类算法将DEGs分为五个聚类。根据京都基因和基因组百科全书(KEGG)数据库对每个簇的功能类别进行了注释;不同的簇与压力后的不同时间点相关联。共有949种代谢物分为10类,脂质和类脂分子占大多数代谢物。差异表达分析揭示了五个时间点中的22种差异调节代谢物(DRMs)。通过RNA-seq和代谢组数据的联合分析,6小时被确定为西瓜抗旱的关键时期,以及淀粉和蔗糖的代谢,植物激素信号转导和光合作用途径被确定为参与西瓜抗旱的重要调控途径。此外,通过联合RNA-seq和代谢组分析结合加权相关网络分析(WGCNA),鉴定了15个与西瓜抗旱相关的候选基因。其中4个基因编码转录因子,包括bHLH(Cla97C03G068160),MYB(Cla97C01G002440),HSP(Cla97C02G033390)和GRF(Cla97C02G042620),ABA途径的一个关键基因,SnRK2-4(Cla97C10G186750),和GP-2基因(Cla97C05G105810),参与淀粉和蔗糖代谢途径。
■总之,本研究为阐明西瓜植物抗旱性的分子机制提供了理论基础,并为该作物的抗旱性研究提供了新的遗传资源。
UNASSIGNED: Watermelon is an annual vine of the family Cucurbitaceae.
Watermelon plants produce a fruit that people love and have important nutritional and economic value. With global warming and deterioration of the ecological environment, abiotic stresses, including drought, have become important factors that impact the yield and quality of watermelon plants. Previous research on
watermelon drought resistance has included analyzing homologous genes based on known drought-responsive genes and pathways in other species.
UNASSIGNED: However, identifying key pathways and genes involved in watermelon drought resistance through high-throughput omics methods is particularly important. In this study, RNA-seq and metabolomic analysis were performed on
watermelon plants at five time points (0 h, 1 h, 6 h, 12 h and 24 h) before and after drought stress.
UNASSIGNED: Transcriptomic analysis revealed 7829 differentially expressed genes (DEGs) at the five time points. The DEGs were grouped into five clusters using the k-means clustering algorithm. The functional category for each cluster was annotated based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database; different clusters were associated with different time points after stress. A total of 949 metabolites were divided into 10 categories, with lipids and lipid-like molecules accounting for the most metabolites. Differential expression analysis revealed 22 differentially regulated metabolites (DRMs) among the five time points. Through joint analysis of RNA-seq and metabolome data, the 6-h period was identified as the critical period for watermelon drought resistance, and the starch and sucrose metabolism, plant hormone signal transduction and photosynthesis pathways were identified as important regulatory pathways involved in watermelon drought resistance. In addition, 15 candidate genes associated with
watermelon drought resistance were identified through joint RNA-seq and metabolome analysis combined with weighted correlation network analysis (WGCNA). Four of these genes encode transcription factors, including bHLH (Cla97C03G068160), MYB (Cla97C01G002440), HSP (Cla97C02G033390) and GRF (Cla97C02G042620), one key gene in the ABA pathway, SnRK2-4 (Cla97C10G186750), and the GP-2 gene (Cla97C05G105810), which is involved in the starch and sucrose metabolism pathway.
UNASSIGNED: In summary, our study provides a theoretical basis for elucidating the molecular mechanisms underlying drought resistance in watermelon plants and provides new genetic resources for the study of drought resistance in this crop.