多环芳烃(PAHs)在环境中分布广泛,对人类健康构成严重威胁。由于其不利的生物学效应和持久性,迫切需要有效降解环境中存在的多环芳烃,尤其是在废水中。在这项研究中,我们获得了一个有效的细菌联盟(PDMC),由Sphingobium属(58.57-72.40%)和假单胞菌属(25.93-39.75%)组成,能够有效利用菲或二苯并噻吩作为唯一碳源。菲培养的财团也可以降解萘,萘,芴,蒽,荧蒽,苯并[a]蒽,二苯并呋喃,咔唑和吲哚,分别。此外,我们确定了上述11种底物的多个关键中间体,并讨论了所涉及的拟议途径。值得注意的是,一种新的中间体1,2-二羟基-4a,9a-二氢蒽-9,10-二酮降解检测,与以前的报道相比,这是极为罕见的。PDMC联盟在添加PAHs混合物的小规模废水生物修复中,在5天内去除100%的PAHs,培养10天后污泥沉降速度为5%。稳定性实验表明,PDMC财团在长达24天的时间内始终具有出色的降解能力。结合微生物多样性分析,结果表明,PDMC联盟是促进PAHs污染环境生物修复的有希望的候选者。
Polycyclic aromatic hydrocarbons (PAHs) are widely distributed in the environment and pose a serious threat to human health. Due to their unfavorable biological effects and persistent properties, it is extremely urgent to effectively degrade PAHs that are present in the environment, especially in wastewater. In this study, we obtained an efficient bacterial consortium (PDMC), consisting of the genera Sphingobium (58.57-72.40%) and Pseudomonas (25.93-39.75%), which is able to efficiently utilize phenanthrene or dibenzothiophene as the sole carbon source. The phenanthrene-cultivated consortium could also degrade naphthalene, acenaphthene, fluorene, anthracene, fluoranthene, benzo[a]anthracene, dibenzofuran, carbazole and indole, respectively. Furthermore, we identified the multiple key intermediates of aforementioned 11 substrates and discussed proposed pathways involved. Notably, a novel intermediate 1,2-dihydroxy-4a,9a-dihydroanthracene-9,10-dione of anthracene degradation was detected, which is extremely rare compared to previous reports. The PDMC consortium removed 100% of PAHs within 5 days in the small-scale wastewater bioremediation added with PAHs mixture, with a sludge settling velocity of 5% after 10 days of incubation. Experiments on the stability reveal the PDMC consortium always has excellent degrading ability for totaling 24 days. Combined with the microbial diversity analysis, the results suggest the PDMC consortium is a promising candidate to facilitate the bioremediation of PAHs-contaminated environments.