简介:MYC转录因子是茉莉酸信号通路的基本调节因子,在植物生长发育和逆境胁迫应答中起重要作用。近年来,新疆核桃主产区冬季严寒和春末霜冻影响了核桃的生长发育,成为制约核桃生产的突出问题。新疆野生核桃是我国仅存的核桃野生树种,其中包含许多具有优秀特征的基因,是重要的栽培和育种。方法:本文,对核桃MYC转录因子成员的理化性质和生物信息学进行分析,并从低温胁迫下的转录组数据中筛选出9个MYC。最后,我们研究了新疆野生核桃中9个MYC成员的亚细胞定位和表达模式。结果:结果显示,从已发表的核桃全基因组数据中鉴定出30名MYC成员,根据聚类分析将它们与拟南芥和杨树的进化关系分为六组,其中JrMYC22和JrMYC23与PtrMYC2b具有较高的同源性,这是由茉莉酸在低温胁迫下诱导的。核桃MYC成员在12条染色体上分布不均。核桃MYC转录因子家族成员启动子顺式作用元件的预测表明,与茉莉酸和低温胁迫相关的顺式作用元件是成员数量最多的元件。与12。此外,新疆野生核桃中所有9个MYC家族成员对低温胁迫的响应均在核中表现出强烈的荧光响应。这些成员响应低温胁迫的表达水平表明JrMYC28,JrMYC31,JrMYC33,JrMYC34和JrMYC35高表达,据推测,JrMYC28,JrMYC31,JrMYC33,JrMYC34和JrMYC35可能在低温胁迫的反应中起关键作用。讨论:本研究结果为进一步研究核桃MYC转录因子家族成员的功能机制提供了理论依据。
Introduction: MYC transcription factors are the basic regulators of the jasmonic acid signaling pathway and play important roles in plant growth and development and the response to adverse stress. In recent years, severe winter freezing and late spring frost in the main planting area of
walnut in Xinjiang have affected the growth and development of walnut, which has become a prominent problem restricting
walnut production. Xinjiang wild
walnut is the only remaining wild species of walnuts in China, which contains a lot of genes with excellent traits, and is important for the cultivation and breeding. Methods: In this paper, the physicochemical properties and bioinformatics of MYC transcription factor members in walnut were analyzed, and the nine MYC were screened from the transcriptome data under low temperature stress. At last, we study the subcellular localizations and the expression patterns of the nine MYC members in Xinjiang wild walnut. Results: The results revealed that 30 MYC members were identified from published
walnut whole-genome data, and their evolutionary relationships with Arabidopsis and poplar were divided into six groups according to clustering analysis, among which JrMYC22 and JrMYC23 had high homology with PtrMYC2b, which is induced by jasmonic acid in response to low-temperature stress.
Walnut MYC members are unevenly distributed on 12 chromosomes. The prediction of promoter cis-acting elements of walnut MYC transcription factor family members revealed that cis-acting elements related to jasmonic acid and lowtemperature stress were the ones with the greatest number of members, with 12. In addition, all nine MYC family members in Xinjiang wild walnut plants responding to low-temperature stress exhibited strong fluorescence responses in the nucleus. The expression levels of these members in response to low-temperature stress revealed that JrMYC28, JrMYC31, JrMYC33, JrMYC34, and JrMYC35 were highly expressed, and it was hypothesized that JrMYC28, JrMYC31, JrMYC33, JrMYC34, and JrMYC35 might play a key role in the response to lowtemperature stress. Discussion: The results of this study provide a theoretical basis for further research on the functional mechanisms of the MYC transcription factor family members in walnut.