背景:可卡因是艾滋病毒感染者[艾滋病毒感染者(PLWH)]中最常被滥用的非法药物之一,它减缓了抗逆转录病毒治疗后病毒产量的下降,并与较高的艾滋病毒载量有关,更快速的艾滋病毒进展,和死亡率增加。
方法:我们研究了可卡因的使用对CD4+T细胞HIV潜伏库(HLR)的影响,美国HIV自然史和治疗史的纵向队列研究.
方法:来自434名不同血统的女性的CD4T细胞基因组DNA(即,75%黑色,14%的西班牙裔,12%的白人)自我报告使用可卡因(即,160名可卡因使用者,59个以前的用户,215个非使用者)使用完整的前病毒HIVDNA分析进行分析,测量每106个CD4+T细胞的完整原病毒。
结果:可卡因使用不同的HIV潜伏储库大小(即,中位数[四分位数范围]:72[14-193]对于从不使用的用户,165[63-387]对于先前的用户,184[28-502]对于当前用户),与从未使用过的可卡因使用者相比,以前(P=0.023)和现在(P=0.001)的可卡因使用者在统计学上都显着更高。
结论:可卡因的使用可能有助于在受病毒学抑制的HIV感染女性中CD4+T细胞中具有更大的复制能力的HLR。我们的发现很重要,因为在HIV病毒库研究和可卡因使用对PLWH结局影响的研究中,女性代表不足。
BACKGROUND: Cocaine-one of the most frequently abused illicit drugs among persons living with HIV [people living with HIV (PLWH)]-slows the decline of viral production after antiretroviral therapy and is associated with higher HIV viral load, more rapid HIV progression, and increased mortality.
METHODS: We examined the impact of cocaine use on the CD4+ T-cell HIV latent reservoir (HLR) in virally suppressed PLWH participating in a national, longitudinal cohort study of the natural and treated history of HIV in the United States.
METHODS: CD4+ T-cell genomic DNA from 434 women of diverse ancestry (ie, 75% Black, 14% Hispanic, 12% White) who self-reported cocaine use (ie, 160 cocaine users, 59 prior users, 215 non-users) was analyzed using the Intact Proviral HIV DNA Assay, measuring intact provirus per 106 CD4+ T cells.
RESULTS: HIV latent reservoir size differed by cocaine use (ie, median [interquartile range]: 72 [14-193] for never users, 165 [63-387] for prior users, 184 [28-502] for current users), which was statistically significantly larger in both prior (P = 0.023) and current (P = 0.001) cocaine users compared with never users.
CONCLUSIONS: Cocaine use may contribute to a larger replication competent HLR in CD4+ T cells among virologically suppressed women living with HIV. Our findings are important because women are underrepresented in HIV reservoir studies and in studies of the impact of cocaine use on outcomes among PLWH.