背景:姜黄(CW)传统上用于治疗由子宫痉挛引起的痛经。然而,食醋加工过程中其成分和抗子宫痉挛的变化以及治疗痛经的机理尚不清楚。
目的:阐明抗子宫痉挛的变化及其物质基础,以及食醋加工前后治疗痛经的机理。
方法:建立子宫痉挛收缩模型,并计算子宫活动及其抑制率以评估差异。采用UPLC-Q-TOF-MS/MS技术快速分析了CW的主要化学成分,并通过多元统计分析探讨了两者之间的差异。然后,通过网络药理学构建与痛经相关的“活性成分-核心靶标-信号通路”的调控网络,并通过分子对接验证了差异活性成分与靶标之间的结合。
结果:CW提取物通过降低收缩张力来放松孤立的子宫,振幅,和频率。与CW相比,醋产品的抑制作用更强,抑制率为70.08%。从CW中鉴定出39种化合物,并筛选出13种差异成分(p<0.05)。网络药理学筛选了11种活性成分和32种潜在靶标,涉及痛经相关的10条关键通路。分子对接结果表明,这些差异活性成分对靶标具有良好的结合活性。
结论:初步揭示CW主要通过调节炎症反应来治疗痛经,通过姜黄酮放松平滑肌和内分泌,13-羟基germacrone,(+)-cuparene,石竹烯氧化物,zederone,和异姜黄烯醇.
BACKGROUND: Curcumae Radix (CW) is traditionally used to treat dysmenorrhea caused by uterine spasm. However, the changes of its composition and anti-uterine spasms during vinegar processing and the mechanism in treating dysmenorrhea are not clear.
OBJECTIVE: To elucidate the changes of anti-uterine spasm and its substance basis, and the mechanism of treating dysmenorrhea before and after vinegar processing.
METHODS: The uterine spasm contraction model was established, and the uterine activity and its inhibition rate were calculated to evaluate the differences. The main chemical constituents of CW were quickly analyzed by UPLC-Q-TOF-MS/MS technology, and the differences between them were explored by multivariate statistical analysis. Then, the regulatory network of \"active ingredients-core targets-signal pathways\" related to dysmenorrhea was constructed by using network pharmacology, and the combination between differential active components and targets was verified by molecular docking.
RESULTS: CW extract relaxed the isolated uterine by reducing the contractile tension, amplitude, and frequency. Compared with CW, the inhibitory effect of vinegar products was stronger, and the inhibition rate was 70.08 %. 39 compounds were identified from CW and 13 differential components were screened out (p<0.05). Network pharmacology screened 11 active components and 32 potential targets, involving 10 key pathways related to dysmenorrhea. The results of molecular docking showed that these differentially active components had good binding activity to target.
CONCLUSIONS: It was preliminarily revealed that CW could treat dysmenorrhea mainly through the regulation of inflammatory reaction, relaxing smooth muscle and endocrine by curcumenone, 13-hydroxygermacrone, (+)-cuparene, caryophyllene oxide, zederone, and isocurcumenol.