■为了研究Siglec10和CD24在正常早期妊娠和稽留流产中的表达,以及它们在母胎界面中的意义。
■对于我们的研究,我们采用Q-PCR和WB技术来评估Siglec10和CD24在非妊娠子宫内膜中的性状和表达,以及正常早期怀孕6-10周的妇女的绒毛和蜕膜以及那些经历过流产的妇女。此外,我们使用ELISA来确定妊娠外周血中Siglec10和CD24的水平,流产错过,和未怀孕的人。采用T检验和方差分析比较各组。
■1.妊娠早期绒毛组织中Siglec10和CD24高表达,稽留流产组表达显著升高(P<0.01)。非妊娠子宫内膜组织中Siglec10和CD24低表达,而早孕蜕膜高表达,在稽留流产中的表达更高(均P<0.05)。正常早期妊娠血清Siglec10和CD24水平明显高于非妊娠(P<0.01)。然而,3.稽留流产组明显高于正常妊娠组(P<0.01)。稽留流产患者血清中CD24的表达随着Siglec10的表达而增加,呈显著正相关(r=0.500,P<0.01)。
■Siglec10和CD24在绒毛中的表达,decidua,与正常妊娠的妇女相比,不明原因的错过流产的外周血上调。这提示血清Siglec10和CD24水平可作为稽留流产的有效预测因子。
UNASSIGNED: To investigate the expression of Siglec10 and CD24 in normal early pregnancy and missed abortion, and their significance in the maternal-fetal interface.
UNASSIGNED: For our research, we employed Q-PCR and WB techniques to evaluate the traits and expression of Siglec10 and CD24 in the nonpregnant endometrium, as well as in the
villus and decidua of women in their 6-10 weeks of normal early pregnancy and those who experienced missed abortion. Additionally, we utilized ELISA to determine the levels of Siglec10 and CD24 in the peripheral blood of pregnancy, missed abortion, and non-pregnant individuals. T-test and ANOVA were used to compare groups.
UNASSIGNED: 1. Villous tissues in early pregnancy showed high expression of Siglec10 and CD24, with a significant increase in expression in the missed abortion group (P < 0.01).2. Nonpregnant endometrial tissue showed low expression of Siglec10 and CD24, while early pregnancy decidua showed high expression, with even higher expression in missed abortion (all P < 0.05).3. Serum levels of Siglec10 and CD24 in normal early pregnancy were significantly higher than non-pregnancy (P < 0.01). However, the missed abortion group showed significantly higher levels than normal pregnancy (P < 0.01).4. CD24 expression in serum of missed abortion increases with Siglec10 expression, indicating a significant positive correlation (r = 0.500, P < 0.01).
UNASSIGNED: Siglec10 and CD24 expression in
villus, decidua, and peripheral blood are up-regulated in unexplained missed abortions than those of women with normal pregnancies. This suggests that the levels of serum Siglec10 and CD24 can be used as an effective predictor of missed abortion.