Mixcoatlus属由三个物种组成:Mixcoatlusbarbouri,M.布朗尼,和黑素瘤,其中黑土分枝杆菌的毒液成分,这三个物种中最常见的物种,直到最近才被描述。然而,对M.barbouri和M.browni的自然史知之甚少,到目前为止,这两个物种的毒液成分仍未被探索。在这项研究中,我们描述了这两种毒液的蛋白质组学特征以及主要的生化和毒性活性。通过对整个毒液的shot弹枪分析获得的蛋白质组学数据确定了M.barbouri的12个蛋白质家族,13分给M.browni.后一种毒液的进一步特征是使用定量的“venomics”方案,这表明它主要由51.1%的磷脂酶A2(PLA2)组成,25.5%蛇毒丝氨酸蛋白酶(SVSP),4.6%L-氨基氧化酶(LAO),和3.6%的蛇毒金属蛋白酶(SVMP),其他六个蛋白质家族的百分比较低。两种毒液均含有crotoxin的碱性和酸性亚基的同源物。然而,由于M.barbouri毒液可获得性的限制,我们只能描述M.browni毒液的类毒素蛋白,我们把它命名为Mixcoatlutoxin.它在小鼠中表现出致命的效力,就像经典响尾蛇蛇毒素所描述的那样。这些发现扩大了对蛇毒蛇类类异二聚体蛋白分布的认识。进一步研究M.barbouri毒液的生物活性,另一方面,仍然是必要的。
The genus Mixcoatlus is composed of three species: Mixcoatlus barbouri, M. browni, and M. melanurus, of which the venom composition of M. melanurus, the most common species of the three, has only recently been described. However, very little is known about the natural history of M. barbouri and M. browni, and the venom composition of these two species has remained thus far unexplored. In this study we characterize the proteomic profiles and the main biochemical and toxic activities of these two venoms. Proteomic data obtained by shotgun analysis of whole venom identified 12 protein families for M. barbouri, and 13 for M. browni. The latter venom was further characterized by using a quantitative \'
venomics\' protocol, which revealed that it is mainly composed of 51.1 % phospholipases A2 (PLA2), 25.5 % snake venom serine proteases (SVSP), 4.6 % l-amino oxidases (LAO), and 3.6 % snake venom metalloproteases (SVMP), with lower percentages other six protein families. Both venoms contained homologs of the basic and acidic subunits of crotoxin. However, due to limitations in M. barbouri venom availability, we could only characterize the crotoxin-like protein of M. browni venom, which we have named Mixcoatlutoxin. It exhibited a lethal potency in mice like that described for classical rattlesnake crotoxins. These findings expand knowledge on the distribution of crotoxin-like heterodimeric proteins in viper snake species. Further investigation of the bioactivities of the venom of M. barbouri, on the other hand, remains necessary.