鹿茸是一种复杂的哺乳动物骨骼器官,具有独特的生物学特性,如再生。快速生长阶段(RGS)是鹿茸再生过程中的一个特殊时期。
为了阐明微小RNA(miRNA)在甘肃马鹿鹿茸发育RGS中的功能,我们使用RNA测序(RNA-seq)分析了三个不同生长阶段鹿茸尖端软骨组织中的miRNA表达谱。
RNA-seq结果揭示了1,073个已知的miRNA和204个新的miRNA,包括30-的1,207、1,242和1,204,60-,和90-d鹿茸软骨组织,分别。为了鉴定控制鹿茸快速生长的关键miRNA,我们在60d内预测了筛选的25个差异表达miRNA(DEM)和特异性表达miRNA(SEM)的靶基因,并注释了它们的功能。KEGG结果显示,25个DEM和30个SEM的靶基因在“代谢途径”中高度分类,“癌症的途径”,“癌症中的蛋白聚糖”和“PI3K-Akt信号通路”。此外,一个新的miRNA(CM008039.1_315920),高度富集的“NF-κB信号通路”,可能需要进一步研究。
在我们的研究中鉴定的miRNA在鹿茸的快速生长中潜在重要。我们的研究结果提供了新的见解,以帮助阐明在关菜鹿茸发育过程中涉及的miRNA介导的调节机制。
The velvet antler is a complex mammalian bone organ with unique biological characteristics, such as regeneration. The rapid growth stage (RGS) is a special period in the regeneration process of velvet antler.
To elucidate the functions of microRNAs (miRNAs) at the RGS of antler development in Gansu red deer (Cervus elaphus kansuensis), we used RNA sequencing (RNA-seq) to analyze miRNA expression profiles in cartilage tissues of deer antler tips at three different growth stages.
The RNA-seq results revealed 1,073 known and 204 novel miRNAs, including 1,207, 1,242, and 1,204 from 30-, 60-, and 90-d antler cartilage tissues, respectively. To identify key miRNAs controlling rapid antler growth, we predicted target genes of screened 25 differentially expressed miRNAs (DEMs) and specifically expressed miRNAs (SEMs) in 60 d and annotated their functions. The KEGG results revealed that target genes of 25 DEMs and 30 SEMs were highly classified in the \"Metabolic pathways\", \"Pathways in cancer\", \"Proteoglycans in cancer\" and \"PI3K-Akt signaling pathway\". In addition, a novel miRNA (CM008039.1_315920), highly enriched in \"NF-kappa B signaling pathway\", may need further study.
The miRNAs identified in our study are potentially important in rapid antler growth. Our findings provide new insights to help elucidate the miRNA-mediated regulatory mechanisms involved during velvet antler development in C. elaphus kansuensis.