■高钠(HS)饮食被认为会影响骨代谢过程。阐明其对钛(Ti)植入物骨整合的影响对植入患者的术后饮食管理具有重要意义。
■这项研究探讨了钠离子(Na)对体内Ti植入物周围新生血管和成骨的影响,利用微型计算机断层扫描,苏木精和伊红染色,和免疫组织化学分析。同时,体外实验评估了不同Na浓度和暴露持续时间对人脐静脉内皮细胞(HUVEC)和MC3T3-E1细胞的影响。
■体内,增加饮食钠(0.8%-6.0%)导致CD34阳性HUVECs和钛植入物周围的新骨形成大幅下降,伴随着炎症细胞的增加。体外,Na+浓度的增加(140-150mmol/L)对增殖产生不利影响,血管生成,和HUVEC的迁移,尤其是长时间接触。虽然MC3T3-E1细胞在短期暴露期间与HUVECs相比最初对高Na+浓度的敏感性较低,长时间暴露于HS环境逐渐减少其增殖,分化,和成骨能力。
■这些发现表明,HS饮食通过干扰术后血管化骨再生的过程,对钛植入物的早期骨整合有负面影响。
UNASSIGNED: A high sodium (HS) diet is believed to affect bone metabolism processes. Clarifying its impact on osseointegration of titanium (Ti) implants holds significant implications for postoperative dietary management of implanted patients.
UNASSIGNED: This investigation probed the impact of sodium ions (Na +) on neovascularization and osteogenesis around Ti implants in vivo, utilizing micro-computed tomography, hematoxylin and eosin staining, and immunohistochemical analyses. Concurrently, in vitro experiments assessed the effects of varied Na + concentrations and exposure durations on human umbilical vein endothelial cells (HUVECs) and MC3T3-E1 cells.
UNASSIGNED: In vivo, increased dietary sodium (0.8%-6.0%) led to a substantial decline in CD34 positive HUVECs and new bone formation around Ti implants, alongside an increase in inflammatory cells. In vitro, an increase in Na + concentration (140-150 mmol/L) adversely affected the proliferation, angiogenesis, and migration of HUVECs, especially with prolonged exposure. While MC3T3-E1 cells initially exhibited less susceptibility to high Na + concentrations compared to HUVECs during short-term exposure, prolonged exposure to a HS environment progressively diminished their proliferation, differentiation, and osteogenic capabilities.
UNASSIGNED: These findings suggest that HS diet had a negative effect on the early osseointegration of Ti implants by interfering with the process of postoperative vascularized bone regeneration.