黏液囊性肿瘤(MCN)是胰腺导管腺癌和肝内胆管癌的前体病变之一。这项研究的目的是通过测量MCN不同成分中的端粒长度来检查短端粒在促进MCN肿瘤发生中的存在。包括粘液衬里上皮,非黏液衬里上皮,和卵巢型基质。共获得45例MCN患者(30例胰腺和15例肝脏)。进行定量端粒特异性荧光原位杂交以测量MCN内特定细胞类型的端粒长度,包括粘液衬里上皮,非黏液衬里上皮,和卵巢型基质,以及正常的导管上皮和腺癌。相对端粒长度在正常导管上皮之间趋于减少,卵巢型基质,非黏液衬里上皮,粘液衬里上皮,和腺癌,不管涉及的器官。在分析的细胞类型中,相对端粒长度在正常导管上皮之间有显著差异(3.31±0.78),卵巢型基质(2.90±0.93),非黏液衬里上皮(2.84±0.79),粘液衬里上皮(2.49±0.93),和腺癌(1.19±0.59),分别(P<0.001,混合效应模型)。不出所料,在正常导管上皮和卵巢型基质之间没有观察到相对端粒长度的差异;然而,在卵巢型基质与卵巢型基质之间的成对比较中观察到显着差异非黏液衬里上皮(P=0.001),非黏液衬里上皮vs.粘液衬里上皮(P=0.005),和粘液衬里上皮vs.腺癌(P<0.001)。这些发现表明,在MCN的肿瘤发生过程中,端粒逐渐缩短。这可能对这种疾病的进展有重要意义。
Mucinous cystic neoplasm (MCN) is one of the precursor lesions of pancreatic ductal adenocarcinoma and intrahepatic cholangiocarcinoma. The aim of this study is to examine the presence of short telomeres in promoting the
tumorigenesis of MCN by measuring telomere lengths in distinct components of MCN, including the mucinous lining epithelium, non-mucinous lining epithelium, and ovarian-type stroma. A total of 45 patients with MCN (30 pancreatic and 15 hepatic cases) were obtained. Quantitative telomere-specific fluorescent in situ hybridization was performed to measure the telomere length of specific cell types within MCNs, including mucinous lining epithelium, non-mucinous lining epithelium, and ovarian-type stroma, as well as normal ductal epithelium and adenocarcinoma. Relative telomere lengths tended to decrease between normal ductal epithelium, ovarian-type stroma, non-mucinous lining epithelium, mucinous lining epithelium, and adenocarcinoma regardless of the involved organs. Among the analyzed cell types, relative telomere lengths were significantly different between normal ductal epithelium (3.31 ± 0.78), ovarian-type stroma (2.90 ± 0.93), non-mucinous lining epithelium (2.84 ± 0.79), mucinous lining epithelium (2.49 ± 0.93), and adenocarcinoma (1.19 ± 0.59), respectively (P < 0.001, mixed-effects model). As expected, no difference in relative telomere lengths was observed between normal ductal epithelium and ovarian-type stroma; however, significant differences were observed in pair-wise comparisons between ovarian-type stroma vs. non-mucinous lining epithelium (P = 0.001), non-mucinous lining epithelium vs. mucinous lining epithelium (P = 0.005), and mucinous lining epithelium vs. adenocarcinoma (P < 0.001). These findings suggest gradual telomere shortening occurs in the
tumorigenesis of MCN, which may have important implications for the progression of this disease.