背景:潜在的传染病和人畜共患风险使得快速诊断皮肤癣菌病在伴侣动物中很重要。
目的:为了比较胶带印象(ATI),纤维图,木灯(WL)检查和真菌培养诊断皮肤癣菌病,以及易于使用的ATI和三坐标图。
方法:五只狗和15只猫有一致的病变,病史和至少两个皮肤癣菌测试结果阳性。
方法:WL检查后,对每位患者的一个代表性病变进行了ATI采样,纤维图和真菌培养。客观和主观地比较了ATI上真菌元素的显微镜检测和样品图。所有测试正确检测皮肤癣菌病的能力,使用Fisher精确检验比较了ATI和三维图像在>20个高倍视野(HPF)中检测真菌元素的能力。
结果:ATI,纤维图,真菌培养和WL在100%(20个中的20个)中呈阳性,90%(20个中的18个),95%(20人中的19人)和65%(20人中的13人)的患者,分别,WL和ATI之间存在显着差异(p=0.0499)。真菌培养显示小孢子菌。canis(n=16),Nannizziagypsea(n=1),毛癣菌(n=1)和红色毛癣菌(n=1)。在>20HPF中,ATI(20个中的18个)检测到真菌元素的频率高于三维图像(20个中的11个)(p=0.017),和检测需要仔细扫描,以区分与背景碎片更经常与三维图像。在ATI上,除了异常的头发碎片外,真菌元素还位于中性粒细胞簇和角质形成细胞筏上。
结论:ATI正确诊断皮肤癣菌病的能力最高,与三维图像相比,更容易检测真菌元素。
BACKGROUND: Potential contagion and zoonotic risk make rapid diagnosis of dermatophytosis important amongst companion animals.
OBJECTIVE: To compare the adhesive tape impression (ATI),
trichogram, Wood\'s lamp (WL) examination and fungal culture for diagnosis of dermatophytosis, and the ATI and
trichogram for ease of use.
METHODS: Five dogs and 15 cats with consistent lesions, history and at least two positive dermatophyte test results.
METHODS: After WL examination, one representative lesion per patient was sampled for ATI, trichogram and fungal culture. Microscopic detection of fungal elements on ATI and trichogram were objectively and subjectively compared. The ability of all tests to correctly detect dermatophytosis, and the ability of ATI and
trichogram to detect fungal elements in >20 high-powered fields (HPF) were compared using Fisher\'s exact tests.
RESULTS: ATI,
trichogram, fungal culture and WL were positive in 100% (20 of 20), 90% (18 of 20), 95% (19 of 20) and 65% (13 of 20) of patients, respectively, with a significant difference between WL and ATI (p = 0.0499). Fungal culture revealed Microsporum. canis (n = 16), Nannizzia gypsea (n = 1), Trichophyton mentagrophytes (n = 1) and T. rubrum (n = 1). Fungal elements were detected in >20 HPF more frequently with ATI (18 of 20) than
trichogram (11 of 20) (p = 0.017), and detection required careful scanning to distinguish from background debris more often with trichogram. Fungal elements were located with neutrophil clusters and keratinocyte rafts in addition to abnormal hair fragments more frequently on ATI.
CONCLUSIONS: ATI had the highest ability to correctly diagnose dermatophytosis, and increased ease of detecting fungal elements compared to trichogram.