寻找控制蜱和蜱传播疾病的目标一直是一个持续存在的问题,到目前为止,我们仍然需要高效,用于此目的的非化学替代品。此搜索必须考虑新的替代方案。例如,基因组学分析是兽医健康研究中用于控制病原体的广泛应用工具。另一方面,我们认为,内分泌机制的调节是生物防治蜱感染的可行替代方案。因此,我们通过RT-PCR在蜱卵巢中进行了一种称为RmERR的微小触毛雌激素相关受体基因的分子鉴定,胚胎细胞,和血淋巴,这使我们能够分析其表达并提出内分泌机制和发育阶段的潜在功能。此外,我们进行了计算机表征,以探索RmERR与不同雌激素的分子相互作用,雌激素拮抗剂,和内分泌干扰物双酚A(BPA),发现通过对接分析预测并由ΔG的负值支持的潜在相互作用(这表明RmERR与评估的分子的潜在相互作用)。此外,系统发育重建表明,RmERR与其他蜱物种归为一组,但在系统发育上与宿主脊椎动物ERR相距遥远。总之,这项研究首次鉴定了牛tickR.microplus中的ERR,并提出了其与不同雌激素的相互作用,支持蜱类中可能的越位调节过程的想法。对这种相互作用及其机制的阐明揭示了其作为开发滴答控制策略的目标的潜力。
The search for targets to control ticks and tick-borne diseases has been an ongoing problem, and so far, we still need efficient, non-chemical alternatives for this purpose. This search must consider new alternatives. For example genomics analysis is a widely applied tool in veterinary health studies to control pathogens. On the other hand, we propose that regulation of endocrine mechanisms represents a feasible alternative to biologically controlling tick infestations. Thus, we performed the molecular identification of an estrogen-related receptor gene of Rhipicephalus microplus called RmERR by RT-PCR in tick ovaries, embryonic cells, and hemolymph, which allowed us to analyze its expression and propose potential functions in endocrine mechanisms and developmental stages. In addition, we performed an in silico characterization to explore the molecular interactions of RmERR with different estrogens, estrogenic antagonists, and endocrine disruptor Bisphenol A (BPA), finding potential interactions predicted by docking analysis and supported by negative values of ΔG (which suggests the potential interaction of RmERR with the molecules evaluated). Additionally, phylogenetic reconstruction revealed that RmERR is grouped with other tick species but is phylogenetically distant from host vertebrates\' ERRs. In summary, this study allowed for the identification of an ERR in cattle tick R. microplus for the first time and suggested its interaction with different estrogens, supporting the idea of a probable
transregulation process in ticks. The elucidation of this interaction and its mechanisms unveiled its potential as a target to develop tick control strategies.