■高强度聚焦超声(HIFU)辅助药物输送是一种将药物输送到目标区域的非侵入性工具,目前主要用于治疗癌症和心血管疾病。然而,在透皮给药方面,HIFU技术仍然知之甚少。因此,这项研究旨在研究HIFU对使用人体皮肤组织的药物渗透进入皮肤的有效性。
■成分为谷胱甘肽的凝胶型药物用异硫氰酸荧光素标记,反过来,在对照组和HIFU治疗组中,使药物在Franz扩散池中渗透至人体皮肤组织24小时,用一个多重酶标仪测量它们的荧光强度,两个,六,和药物应用后24小时。此外,在药物施用后24小时对每个组织切片进行组织切片分析.%面积,单位面积的荧光强度,使用荧光显微镜测量药物的穿透深度。
■所有组中的荧光强度随时间增加。具体来说,在药物应用后24小时,荧光强度(a.u).与对照组相比,10次注射HIFU治疗组显着增强(p<0.05)。组织切片分析显示,与对照组相比,两个HIFU治疗组的荧光药物面积%和单位面积的荧光强度(a.u.)均显著增加(p<0.05,p<0.001)。此外,与对照组相比,两个HIFU治疗组的渗透深度(μm)也显着增加(p<0.01,p<0.05)。
■首次证明HIFU显着促进局部药物渗透到人体皮肤中,强烈暗示HIFU可以是透皮给药的有用选择。
UNASSIGNED: High-intensity focused ultrasound (HIFU)-assisted drug delivery is a non-invasive tool to deliver drugs to targeted areas, currently used mainly for treating cancer and cardiovascular diseases. However, in terms of transdermal drug delivery, HIFU technology is still poorly understood. Accordingly, this study sought to investigate the effectiveness of HIFU on drug penetration into the skin using human skin tissues.
UNASSIGNED: Gel-type drugs whose ingredient is glutathione were labelled with fluorescein isothiocyanate, in turn the drugs were allowed to penetrate to the human skin tissue in the Franz diffusion cell for 24 hours in control and HIFU treatment groups, and their fluorescence intensity was measured using a multiple microplate reader at one, two, six, and 24 hours after drug application. In addition, tissue slice analysis was performed in each tissue slice at 24 hours post-drug application. The % area, fluorescence intensity per area, and penetration depth of the drug were measured using a fluorescence microscope.
UNASSIGNED: The fluorescence intensity increased with time in all groups. Specifically, at 24 hours after drug application, the fluorescence intensity (a.u). of the 10-shot HIFU treatment group was significantly enhanced compared to that of the control group (p < 0.05). The tissue slice analysis demonstrated that the % area of fluorescent drug and the fluorescence intensity per area (a.u.) were all significantly increased in both HIFU treatment groups compared to the control group (p < 0.05, p < 0.001). In addition, the penetration depth (μm) also markedly rose in both HIFU treatment groups compared to the control group (p < 0.01, p < 0.05).
UNASSIGNED: It was demonstrated for the first time that HIFU significantly facilitated topical drug penetration into the human skin, strongly implying that HIFU can be a useful option for transdermal drug delivery.