目的:本研究的目的是评估酸性饮料对人牙齿表面形貌和元素组成的影响。
方法:总共五种高酸性饮料(红牛,百事可乐,苹果Cidra,唐·莫桑比,和唐橙)进行了调查。将实验组的牙齿标本浸入每种饮料中,并在37°C下孵育7天,然而,对照组的牙齿标本置于蒸馏水中。之后,使用扫描电子显微镜(SEM)分析牙齿样本,立体显微镜,和能量色散X射线(EDX)技术。
结果:与对照组相比,所有实验组的牙齿元素都有所下降,然而,这种下降没有统计学意义。然而,比较实验组,与百事可乐相比,红牛饮料导致钙和磷元素的百分比显着降低,苹果Cidra,唐·莫桑比,和唐橙饮料,但与对照饮料相比,它也微不足道。在SEM分析下,所有五种酸性饮料都显示出侵蚀潜力;然而,每组标本显示出不同程度的脱矿质。此外,与各自的对照样本相比,所有实验组的牙齿样本均表现出明显的变色。
结论:在研究的局限性内,在SEM分析下,所有五种酸性饮料在模拟体外条件下都表现出侵蚀潜力;然而,每组标本表现出不同程度的脱矿质。此外,在EDX分析下,所有饮料的总体效果均不显著,因为实验组和对照组标本的元素组成之间没有实质性差异。
OBJECTIVE: The objective of this study was to evaluate the effect of acidic beverages on the surface topography and elemental composition of human teeth.
METHODS: A total of five highly acidic beverages (Red Bull, Pepsi, Apple Cidra, Tang Mosambi, and Tang Orange) were investigated. The tooth specimens of experimental groups were submerged in each beverage and incubated at 37 °C for 7 days, whereas, the tooth specimens of control groups were placed in distilled water. Afterwards, tooth specimens were analyzed using scanning electron microscopic (SEM), stereomicroscopic, and energy dispersive x-ray (EDX) techniques.
RESULTS: All experimental groups revealed a decline in the tooth elements compared to controls, however, such decline was not statistically significant. Nevertheless, comparing the experimental groups, the Red Bull beverage caused a marked reduction in the percentage of both calcium and phosphorus elements compared to the Pepsi, Apple Cidra, Tang Mosambi, and Tang Orange beverages but it was insignificant as well in contrast to its control counterpart. All five acidic beverages demonstrated erosive potential under SEM analysis; however, each group of specimens showed a diverse amount of demineralization. In addition, all experimental groups exhibited significant discoloration of tooth specimens compared to their respective control counterparts.
CONCLUSIONS: Within the limitations of study, all five acidic beverages demonstrated erosive potential in the simulated in vitro conditions under SEM analysis; however, each group of specimens exhibited a different extent of demineralization. In addition, the overall effect of all beverages was insignificant under EDX analysis as no substantial difference was revealed between the elemental composition of experimental and control group specimens.