背景:钛-铝-钒合金(Ti-6Al-4V)由于其生物相容性而经常用于植入。使用3D打印可以对植入物结构进行机械修改,并使其形状适应个体患者的特定需求。
方法:使用3DCAD方法设计钛合金板,并使用3DSLM打印机打印。使用微型计算机断层扫描扫描仪对材料表面进行定性测试。使用MTT测定法对L929细胞系并与Balb/3T3细胞直接接触,研究模块化钛板的细胞毒性。用hFOB1.19人成骨细胞评估细胞对材料表面的粘附。在鼠李糖乳杆菌菌株上研究了微生物生物膜的形成,表皮葡萄球菌,变形链球菌和白色念珠菌使用TTC测试和扫描电子显微镜(SEM)。
结果:表面分析显示植入物的疏水性。研究表明,钛板没有细胞毒性。此外,材料表面表现出良好的成骨细胞粘附性能。在测试的微生物中,变形链球菌和表皮葡萄球菌菌株对平板表面的粘附能力最高,而真菌白色念珠菌显示出最低的粘附能力。
结论:制造的模块化板具有有利于植入和减少选定形式的微生物生物膜的特性。三维打印模块化钛板在这项研究中进行了研究,并揭示了这种材料的潜在临床应用,关于缺乏细胞毒性,成骨细胞的高粘附性和生物膜形成的减少。3DCAD方法允许我们为个体患者个性化植入物的形状。
BACKGROUND: The titanium-aluminum-vanadium alloy (Ti-6Al-4V) is frequently used in implantology due to its biocompatibility. The use of 3D printing enables the mechanical modification of implant structures and the adaptation of their shape to the specific needs of individual patients.
METHODS: The titanium alloy plates were designed using the 3D CAD method and printed using a 3D SLM printer. Qualitative tests were performed on the material surface using a microcomputed tomography scanner. The cytotoxicity of the modular titanium plates was investigated using the MTT assay on the L929 cell line and in direct contact with Balb/3T3 cells. Cell adhesion to the material surface was evaluated with hFOB1.19 human osteoblasts. Microbial biofilm formation was investigated on strains of Lactobacillus rhamnosus, Staphylococcus epidermidis, Streptococcus mutans and Candida albicans using the TTC test and scanning electron microscopy (SEM).
RESULTS: The surface analysis showed the hydrophobic nature of the implant. The study showed that the titanium plates had no cytotoxic properties. In addition, the material surface showed favorable properties for osteoblast adhesion. Among the microorganisms tested, the strains of S. mutans and S. epidermidis showed the highest adhesion capacity to the plate surface, while the fungus C. albicans showed the lowest adhesion capacity.
CONCLUSIONS: The manufactured modular plates have properties that are advantageous for the implantation and reduction in selected forms of microbial biofilm. Three-dimensional-printed modular titanium plates were investigated in this study and revealed the potential clinical application of this type of materials, regarding lack of cytotoxicity, high adhesion properties for osteoblasts and reduction in biofilm formation. The 3D CAD method allows us to personalise the shape of implants for individual patients.