背景:昆虫遇到各种环境压力,响应它们产生活性氧(ROS)。超氧化物歧化酶(SOD)是一种抗氧化金属酶,可清除超氧化物自由基以防止氧化损伤。
目的:研究黄粉虫在氧化应激条件下SODs的表达。
方法:这里,我们研究了黄粉菌中农药和重金属对SODs的转录表达。首先,我们在RNA-Seq数据库中搜索了T.molitorSOD(TmSOD)基因,并鉴定了两种SOD亚型(TmSOD1-iso1和iso2)。我们检查了他们在发育阶段的活动,组织特异性,和各种类型(农药和重金属)的氧化应激使用qPCR。
结果:我们的结果揭示了两种新形式的TmSOD。这些TmSOD具有铜/锌超氧化物歧化酶结构域,活动站点,Cu2+结合位点,Zn2+结合位点,E类二聚体接口,和P类二聚体界面。TmSODs(TmSOD1-iso1和iso2)在不同的发育阶段和组织中表达。农药和重金属引起这些TmSOD的上调。
结论:我们的研究结果表明,这两种TmSOD在墨虫中具有不同的功能,为墨虫的解毒能力提供见解。
BACKGROUND: Insects encounter various environmental stresses, in response to which they generate reactive oxygen species (ROS). Superoxide dismutase (SOD) is an antioxidant metalloenzyme that scavenges superoxide radicals to prevent oxidative damage.
OBJECTIVE: To investigate expressions of SODs under oxidative stress in Tenebrio molitor.
METHODS: Here, we investigated the transcriptional expression of SODs by pesticide and heavy metals in Tenebrio moltior. First, we searched an RNA-Seq database for T. molitor SOD (TmSOD) genes and identified two SOD isoforms (TmSOD1-iso1 and iso2). We examined their activities under developmental stage, tissue-specific, and various types (pesticide and heavy metal) of oxidative stress by using qPCR.
RESULTS: Our results revealed two novel forms of TmSODs. These TmSODs had a copper/zinc superoxide dismutase domain, active site, Cu2+ binding site, Zn2+ binding site, E-class dimer interface, and P-class dimer interface. TmSODs (TmSOD1-iso1 and iso2) were expressed in diverse developmental phases and tissues. Pesticides and heavy metals caused an upregulation of these TmSODs.
CONCLUSIONS: Our findings suggest that the two TmSODs have different functions in T. molitor, providing insights into the detoxification ability of T. molitor.