Acuticoccussp.I52.16.1是从印度洋100m水深中分离出来的,并介绍了一种属于针叶树科的新型针叶树属,类变形杆菌。菌株I52.16.1表现出硝酸还原酶的活性,亚硝酸还原酶,和脲酶。Acuticoccussp.的基因组。I52.16.1由环状染色体(5,134,086bp)组成,GC含量为69.7mol%。预测编码基因的数量为4935个,包括6个rRNA,47tRNA,和2个sRNA。16SrDNA序列与扬子针菌JL1095T最大相似度为97.58%,其次是墨红球菌PTG4-2T(97.05%),红叶针菌J103T(96.52%),红树林针菌B2012T(95.85%)。Acuticoccussp.I52.16.1包含与同化铵有关的基因进化枝,硝酸盐,亚硝酸盐,和简单的有机氮化合物,但缺乏异化反硝化途径。还检测到两种不同类型的脲酶,提示遗传异质性。这项研究提供了对贫营养海洋表面异养细菌氮代谢策略的见解。
Acuticoccus sp. I52.16.1 was isolated from 100 m water depth from the Indian Ocean, and presented a novel Acuticoccus species belonging to the family Acuticoccaceae, class Alphaproteobacteria. The strain I52.16.1 displayed the activities of nitrate reductase, nitrite reductase, and urease. The genome of Acuticoccus sp. I52.16.1 consisted of a circular chromosome (5, 134, 086bp) with a G + C content of 69.7 mol%. The predicted number of coding genes was 4935, including 6 rRNA, 47 tRNA, and 2 sRNA. The 16S rDNA sequence displayed the maximum similarity of 97.58% with Acuticoccus yangtzensis JL1095T, followed by Acuticoccus sediminis PTG4-2T (97.05%), Acuticoccus kandeliae J103T (96.52%), and Acuticoccus mangrove B2012T (95.85%). Acuticoccus sp. I52.16.1 contained clades of genes involved in assimilating ammonium, nitrate, nitrite, and simple organic nitrogen compounds, but lacked the pathway for dissimilatory denitrification. Two distinct types of ureases were also detected, suggesting genetic heterogeneity. This study provided insight into the nitrogen metabolism strategies of heterotrophic bacteria in the oligotrophic ocean surface.