未经处理的印染废水不受控制地释放到水生生态系统中会带来全球环境风险。它改变了本地微生物群落和相关的生态过程,经常被忽视。因此,使用水沉积物微观世界研究了酸性橙7染料(AO7)污染对天然微生物群落的影响。与无菌的缩影相比,在自然的微观世界中完全的染料脱色显示了微生物群落在对抗外源性生物污染方面的意义。变形杆菌主导了水群落,而Firmicutes主导了沉积物。AO7暴露会引起水和沉积物中细菌群落结构组成的显着变化。Niveispirillum表现出明显的下降,假单胞菌显着增加。Niveispirillum中的-9.0log2FC,一种固氮细菌,从对照组的24.4%到治疗后的0.1%,可能会破坏营养平衡,植物生长,和生态系统生产力。相反,假单胞菌的水平升高。偶氮染料暴露的结果证明了其耐受和生物有机污染物的能力,强调其韧性。通过KEGG途径分析的功能谱分析揭示了AO7胁迫下的差异表达模式。具体来说,缬氨酸,亮氨酸,水中异亮氨酸降解途径减少了52.2%,半胱氨酸和蛋氨酸代谢完全停止表达,表明染料暴露下蛋白质代谢和营养生物利用度降低。此外,在沉积物中,谷胱甘肽代谢停止,表明AO7输注后氧化应激增加。然而,C5-支链二元酸的代谢以及柠檬烯和pine烯的降解在沉积物中独特地表达。甲烷代谢的减少加剧了全球变暖对水生生态系统的影响。Further,停止的丁酸代谢途径反映了纺织染料废水对生态过程的不利影响,如有机物分解,能量流,营养循环,和社区动态,有助于维持河流生态系统的自我净化和生态平衡。这些发现强调了迫切需要更全面的环境监测和管理策略,以减轻水生生态系统中纺织染料带来的生态风险。仍然没有被注意到。
The uncontrolled release of untreated dyeing wastewater into aquatic ecosystems poses global environmental risks. It alters native microbial communities and associated ecological processes, often going unnoticed. Therefore, the influence of acid orange 7 dye (AO7) contamination on the natural microbial community was investigated using a water-sediment microcosm. Compared to sterile microcosms, complete dye decolourization in natural microcosms showed microbial communities\' significance in combating xenobiotic contamination. Proteobacteria dominated the water community, whereas Firmicutes dominated the sediment. AO7 exposure induced notable shifts in the structural composition of the bacterial community in both water and sediment. Niveispirillum exhibited a marked decrease, and Pseudomonas demonstrated a notable increase. The - 9.0 log2FC in Niveispirillum, a nitrogen-fixing bacterium, from 24.4% in the control to 0.1% post-treatment, may disrupt nutrient balance, plant growth, and ecosystem productivity. Conversely, elevated levels of Pseudomonas sp. resulting from azo dye exposure demonstrate its ability to tolerate and bioremediate organic pollutants, highlighting its resilience. Functional profiling via KEGG pathway analysis revealed differential expression patterns under AO7 stress. Specifically, valine, leucine, and isoleucine degradation pathways in water decreased by 52.2%, and cysteine and methionine metabolism ceased expression entirely, indicating reduced protein metabolism and nutrient bioavailability under dye exposure. Furthermore, in sediment, glutathione metabolism ceased, indicating increased oxidative stress following AO7 infusion. However, C5-branched dibasic acid metabolism and limonene and pinene degradation were uniquely expressed in sediment. Decreased methane metabolism exacerbates the effects of global warming on aquatic ecosystems. Further, ceased-butanoate metabolic pathways reflect the textile dye wastewater-induced adverse impact on ecological processes, such as organic matter decomposition, energy flow, nutrient cycling, and community dynamics that help maintain self-purification and ecological balance in river ecosystems. These findings underscore the critical need for more comprehensive environmental monitoring and management strategies to mitigate ecological risks posed by textile dyes in aquatic ecosystems, which remain unnoticed.