开发并研究了无试剂电化学葡萄糖生物传感器。用电化学合成的树枝状金纳米结构(DGNs)和氧化还原介体(Med)如二茂铁羧酸(FCA)修饰的石墨棒(GR)电极,1,10-菲罗啉-5,6-二酮(PD),N,N,N\',N'-四甲基联苯胺(TMB)或四硫富瓦烯(TTF)与葡萄糖氧化酶(GOx)(GR/DGNs/FCA/GOx,GR/DGNs/PD/GOx,GR/DGNs/TMB/GOx,或GR/DGNs/TTF/GOx)进行了开发和电化学研究。发现基于三层逐层沉积的PD和GOx(GR/DGNs/(PD/GOx)3)的生物传感器最适合测定葡萄糖。为了提高所开发的生物传感器的性能,用聚吡咯(Ppy)修饰GR/DGNs/(PD/GOx)3电极的表面5h。基于GR/DGNs/(PD/GOx)3/Ppy(5h)电极的葡萄糖生物传感器使用高达39.0mmolL-1的葡萄糖的宽线性动态范围进行表征,对于29.4mmolL-1葡萄糖浓度,灵敏度为3.03µAmM-1cm-2,检出限为0.683mmolL-1,重复性为9.03%。基于Ppy的葡萄糖生物传感器的特征在于良好的储存稳定性(τ1/2=9.0天)。此外,研究了开发的生物传感器在血清中的性能。
Reagentless electrochemical glucose biosensors were developed and investigated. A graphite rod (GR) electrode modified with electrochemically synthesized dendritic gold nanostructures (DGNs) and redox mediators (Med) such as ferrocenecarboxylic acid (FCA), 1,10-phenathroline-5,6-dione (PD), N,N,N\',N\'-tetramethylbenzidine (TMB) or
tetrathiafulvalene (TTF) in combination with glucose oxidase (GOx) (GR/DGNs/FCA/GOx, GR/DGNs/PD/GOx, GR/DGNs/TMB/GOx, or GR/DGNs/TTF/GOx) were developed and electrochemically investigated. A biosensor based on threefold-layer-by-layer-deposited PD and GOx (GR/DGNs/(PD/GOx)3) was found to be the most suitable for the determination of glucose. To improve the performance of the developed biosensor, the surface of the GR/DGNs/(PD/GOx)3 electrode was modified with polypyrrole (Ppy) for 5 h. A glucose biosensor based on a GR/DGNs/(PD/GOx)3/Ppy(5 h) electrode was characterized using a wide linear dynamic range of up to 39.0 mmol L-1 of glucose, sensitivity of 3.03 µA mM-1 cm-2, limit of detection of 0.683 mmol L-1, and repeatability of 9.03% for a 29.4 mmol L-1 glucose concentration. The Ppy-based glucose biosensor was characterized by a good storage stability (τ1/2 = 9.0 days). Additionally, the performance of the developed biosensor in blood serum was investigated.