■三级淋巴结构(TLS),肿瘤免疫微环境(TIME)中肿瘤浸润免疫细胞的有序结构,在各种癌症的发展和抗肿瘤免疫中起重要作用,包括肝脏,结肠,和胃癌。先前的研究表明,在肿瘤内(IT)中存在TLS,侵入性切缘(IM),和肿瘤在各种成熟状态下的肿瘤周围(PT)区域。然而,非小细胞肺癌(NSCLC)不同区域的TLS密度尚未得到广泛研究.
■使用免疫组织化学(IHC)染色在82例NSCLC患者中评估TLS和肿瘤浸润性免疫细胞。肿瘤样本分为三个亚区域作为IT,IM和PT区域,和TLS被确定为早期/原发性TLS(E-TLS)或继发性/卵泡TLS(F-TLS)。TLS在不同到期状态下的分布,以及它们与临床病理特征和预后价值的相关性,被评估。列线图用于预测1-,3-,非小细胞肺癌患者的5年总生存期(OS)。
■在IT地区,TLS的密度和F-TLS的比例(90.2%,0.45/mm2,61.0%,分别)显著高于IM地区(72.0%,0.18/mm2,39.0%,分别)和PT区域(67.1%,0.16/mm2,40.2%,分别)。较低密度的TLS,尤其是IM区域的E-TLS,非小细胞肺癌患者预后较好。CD20+B细胞,CD3+T细胞,CD8+细胞毒性T细胞,CD68+巨噬细胞在IM区显著过表达。IM区CD20+B细胞和CD3+T细胞与E-TLS密度显著相关,而与F-TLS无统计学意义的相关性。IM区E-TLS密度和TNM分期是NSCLC患者的独立预后因素。列线图显示出良好的预后能力。
■在非小细胞肺癌患者中,IM区域E-TLS的密度越高,预后越差。可能是由于肿瘤侵袭前沿的抑制性免疫细胞密度增加引起的TLS成熟抑制。
UNASSIGNED: Tertiary lymphoid structures (TLS), ordered structure of tumor-infiltrating immune cells in tumor immune microenvironment (TIME), play an important role in the development and anti-tumor immunity of various cancers, including liver, colon, and gastric cancers. Previous studies have demonstrated that the presence of TLS in intra-tumoral (IT), invasive margin (IM), and peri-tumoral (PT) regions of the tumors at various maturity statuses. However, the density of TLS in different regions of non-small cell lung cancer (NSCLC) has not been extensively studied.
UNASSIGNED: TLS and tumor-infiltrating immune cells were assessed using immunohistochemistry (IHC) staining in 82 NSCLC patients. Tumor samples were divided into three subregions as IT, IM and PT regions, and TLS were identified as early/primary TLS (E-TLS) or secondary/follicular TLS (F-TLS). The distribution of TLS in different maturity statuses, along with their correlation with clinicopathological characteristics and prognostic value, was assessed. Nomograms were used to predict the probability of 1-, 3-, and 5-year overall survival (OS) in patients with NSCLC.
UNASSIGNED: The density of TLS and proportion of F-TLS in the IT region (90.2%, 0.45/mm2, and 61.0%, respectively) were significantly higher than those in the IM region (72.0%, 0.18/mm2, and 39.0%, respectively) and PT region (67.1%, 0.16/mm2, and 40.2%, respectively). A lower density of TLS, especially E-TLS in the IM region, was correlated with better prognosis in NSCLC patients. CD20+ B cells, CD3+ T cells, CD8+ cytotoxic T cells, and CD68+ macrophages were significantly overexpressed in the IM region. CD20+ B cells and CD3+ T cells in the IM region were significantly correlated with the density of E-TLS, while no statistically significant correlation was found with F-TLS. The E-TLS density in the IM region and TNM stage were independent prognostic factors for NSCLC patients. The nomogram showed good prognostic ability.
UNASSIGNED: A higher density of E-TLS in the IM region was associated with a worse prognosis in NSCLC patients, potentially due to the inhibition of TLS maturation caused by the increased density of suppressive immune cells at the tumor invasion front.