目的:一些临床和实验研究表明,L-精氨酸,具有抗氧化特性,加速组织愈合。这项研究检查了口服L-精氨酸补充对Wistar大鼠肌腱再生的体内作用。
方法:对于平均250-300g的每个权重,将24只Wistar年夜鼠等分为三组。将每只大鼠的右后腿跟腱切除,然后修复。第一组(对照)用标准食物和水的方案随访。在第二组(L-Arg低剂量)中,300mg/kg,在第三组(L-Arg高剂量)中,每天以标准食物和水的方案在水中施用600mg/kg的L-精氨酸。八周后,老鼠被处死,并对肌腱进行了组织学和生物力学分析。
结果:L-Arg低剂量组和L-Arg高剂量组的肌腱峰强度值相似,但明显高于对照组。在地面物质方面,两组之间观察到统计学上的显着差异,光纤排列,cellularity,透明质化,和GAG性质(p=0.05,p=0.002,p=0.016,p=0.027,p=0.05)。根据胶原性质的组织学检查,组间无统计学差异,纤维结构,腱细胞特性,原子核的圆化,和胶原蛋白可染色性。(p=0.999,p=0.061,p=0.195,p=0.195,p=0.130)。没有死亡,伤口并发症,或观察到重新破裂。
结论:与对照组相比,确定了补充L-精氨酸对肌腱愈合的组织学和生物力学上不同的治疗效果。
方法:5.
OBJECTIVE: Several clinical and experimental studies have revealed that L-Arginine, which has antioxidant properties, accelerates tissue healing. This study examined the in vivo effects of oral L - Arginine supplementation on tendon regeneration in Wistar rats.
METHODS: For each weighting of an average of 250-300 g, 24 Wistar rats were separated into three equal groups. Each rat\'s right hind leg Achilles tendons were tenotomized and then repaired. The first group (Control) was followed up with a regimen of standard food and water. In the second group (L-Arg Low Dose), 300 mg/kg, and in the third group (L-Arg High Dose), 600 mg/kg L-Arginine was administered in water daily with a regimen of standard food and water ad libitum. After eight weeks, the rats were sacrificed, and the tendons were histologically and biomechanically analyzed.
RESULTS: Tendon peak strength values of the L-Arg Low Dose and L-Arg High Dose groups were similar but significantly higher than the control group. A statistically significant difference was observed between the groups in terms of ground substance, fiber arrangement, cellularity, hyalinization, and GAG properties ( p = 0.05, p = 0.002, p = 0.016, p = 0.027, p = 0.05). There was no statistically significant difference between the groups according to the histological examination of collagen properties, fiber structure, tenocyte properties, rounding of the nuclei, and collagen stainability. (p = 0.999, p = 0.061, p = 0.195, p = 0.195, p = 0.130). No mortality, wound complications, or re-ruptures were observed.
CONCLUSIONS: Compared with the control group, histologically and biomechanically distinct therapeutic effects of L-Arginine supplementation on tendon healing were determined.
METHODS: 5.