本文采用区带电泳,分子生物学中最常用的工具之一,探索汉斯-约尔格·莱茵伯格对实验的反思中得出的两个想法。首先,技术对象-仪器和物质条件-在知识或认识事物的生产中发挥的约束作用。第二,通过这些技术对象生产相互连接的实验系统,这导致了研究领域和实验文化的意外纠缠。到1960年代初,世界各地实验室区带电泳的开始发生了一些变化——有人说,彻底改变了蛋白质的研究。即使在今天,电泳继续开放生物医学的研究场所和问题,分子生物学,人类遗传学,在分子进化领域。在我的文章中,我试图研究区带电泳的相互联系的生活,并解决更广泛的社会问题,甚至是全球背景,在其中,这种看似不起眼的技术成为生产生物知识的重要工具。这样做,我的目标是将实验系统的历史和史学的过去和现在带到未来,实验和技术在不同的地理和环境中使用时受到质疑,包括贫困的背景。
This paper uses zone electrophoresis, one of the most frequently used tools in molecular biology, to explore two ideas derived from Hans-Jörg Rheinberger\'s reflections on experiments. First, the constraining role played by technical objects-instrumentation and material conditions-in the production of knowledge or epistemic things. Second, the production of interconnected experimental systems by such technical objects, which results in the unexpected entanglement of research fields and experimental cultures. By the beginning of the 1960s, the inception of zone electrophoresis in laboratories around the world transformed-some say, revolutionized-the study of proteins. Even today, electrophoresis continues to open research venues and questions in biomedicine, molecular biology, human genetics, and in the field of molecular evolution. In my essay, I seek to look at the interconnected lives of zone electrophoresis and address the broader social, and even global context, in which this apparently humble technique became a salient tool in the production of biological knowledge. In so doing, I aim to take the past and present of the history and historiography of experimental systems to the future, where experiments and technologies are interrogated as they are used in different geographies and contexts, including contexts of poverty.