■蛋白质组学提供了检测和监测神经性厌食症(AN)及其变体的潜力,非典型AN(atyp-AN)。然而,研究受到小型蛋白质面板的限制,专注于成人AN,缺乏复制。
■在这项研究中,我们对患有AN/atyp-AN的女性(n=64)的92种炎症相关蛋白进行了Olink多重分析,所有这些人都≤预期体重的90%,和年龄匹配的健康对照个体(n=44)。
■五种蛋白质在原发性AN/atyp-AN组和健康对照组之间存在显着差异(较低水平:HGF,IL-18R1,TRANCE;更高水平:CCL23,LIF-R)。在我们的主要模型中,在患有AN的参与者中,3种蛋白质(较低的IL-18R1,TRANCE;较高的LIF-R)的表达水平被独特地破坏。atyp-AN没有出现独特的表达水平。在总样本中,12种蛋白质(ADA,CD5,CD6,CXCL1,FGF-21,HGF,IL-12B,IL18,IL-18R1,SIRT2,TNFSF14,TRANCE)与体重指数和5种蛋白(CCL11,FGF-19,IL8,LIF-R,在我们的主要模型中,OPG)与体重指数呈负相关。
■我们的结果复制了先前研究的结果,该研究表明AN中炎症状态失调,并将这些结果扩展到atyp-AN。在与体重指数相关的17种蛋白质中,11是从以前使用类似方法的研究中复制的,强调炎症蛋白表达水平作为AN疾病监测生物标志物的前景。我们的发现强调了AN和atyp-AN的复杂性,强调了鉴定的蛋白质无法区分这两种亚型。从而强调了这些疾病的异质性。
我们检查了患有神经性厌食症(AN)和非典型AN的青春期女孩中的73种炎症蛋白,并将其与年龄匹配的健康对照女孩进行了比较。发现了显著的差异,由5种关键蛋白质驱动(较低:HGF,IL-18R1,TRANCE;更高:CCL23,LIF-R)。三种蛋白质(TRANCE,LIF-R,IL-18R1)将具有AN的低体重参与者与对照参与者进行了唯一区分。我们的研究揭示了AN和非典型AN中不同的炎症模式,并揭示了这些疾病背后潜在的状态特异性因素。
UNASSIGNED: Proteomics offers potential for detecting and monitoring anorexia nervosa (AN) and its variant, atypical AN (atyp-AN). However, research has been limited by small protein panels, a focus on adult AN, and lack of replication.
UNASSIGNED: In this study, we performed Olink multiplex profiling of 92 inflammation-related proteins in females with AN/atyp-AN (n = 64), all of whom were ≤90% of expected body weight, and age-matched healthy control individuals (n = 44).
UNASSIGNED: Five proteins differed significantly between the primary AN/atyp-AN group and the healthy control group (lower levels: HGF, IL-18R1, TRANCE; higher levels: CCL23, LIF-R). The expression levels of 3 proteins (lower IL-18R1, TRANCE; higher LIF-R) were uniquely disrupted in participants with AN in our primary model. No unique expression levels emerged for atyp-AN. In the total sample, 12 proteins (ADA, CD5, CD6, CXCL1, FGF-21, HGF, IL-12B, IL18, IL-18R1, SIRT2, TNFSF14, TRANCE) were positively correlated with body mass index and 5 proteins (CCL11, FGF-19, IL8, LIF-R, OPG) were negatively correlated with body mass index in our primary models.
UNASSIGNED: Our results replicate the results of a previous study that demonstrated a dysregulated inflammatory status in AN and extend those results to atyp-AN. Of the 17 proteins correlated with body mass index, 11 were replicated from a previous study that used similar methods, highlighting the promise of inflammatory protein expression levels as biomarkers of AN disease monitoring. Our findings underscore the complexity of AN and atyp-AN by highlighting the inability of the identified proteins to differentiate between these 2 subtypes, thereby emphasizing the heterogeneous nature of these disorders.
We examined 73 inflammation proteins in adolescent girls with anorexia nervosa (AN) and atypical AN and compared them with age-matched healthy control girls. Significant differences were found, driven by 5 key proteins (lower: HGF, IL-18R1, TRANCE; higher: CCL23, LIF-R). Three proteins (TRANCE, LIF-R, IL-18R1) uniquely distinguished low-weight participants with AN from control participants. Our study reveals distinct inflammation patterns in AN and atypical AN and sheds light on potential state-specific factors that underlie these disorders.