背景:及时诊断弓形虫感染是预防和控制弓形虫病传播的必要条件。金免疫层析法(GICA)是一种快速检测样品中病原体的方法。已经开发了基于GICA的诊断方法,以高灵敏度和特异性准确检测病原体,在弓形虫诊断中的应用有望取得良好的效果。
方法:使用弓形虫C末端截短的顶端膜抗原1(AMA1C)制作胶体金试纸。在最佳条件下合成了胶体金-AMA1C和胶体金-鼠蛋白缀合物。用AMA1C和山羊抗小鼠抗体处理硝酸纤维素膜作为测试线和对照线,分别。总的来说,使用AMA1C-GICA和商业酶联免疫吸附测定(ELISA)试剂盒测试了90只猫血清样品。使用便携式胶体金免疫层析试纸条分析仪(HMREADER)数字显示GICA结果。敏感性,特异性,并对AMA1C-GICA的稳定性进行了评估,然后用于检查临床样本,包括203人血清,266猫血清,和81只狗血清。
结果:AMA1C-GICA对弓形虫阳性血清的检测阈值为1:32。GICA试纸条特异性检测弓形虫抗体,与间日疟原虫无反应,科利科蒂并,日本血吸虫,华支睾吸虫,还有曼氏血吸虫.因此,对于每个测定,使用AMA1C-GICA和商业ELISA试剂盒检测到15个(16.7%)阳性样品。受试者工作特征曲线显示GICA的相对灵敏度为85.3%,特异性为92%,曲线下面积为98%。在使用HMREADER分析临床样本后,发现这些样品中有1.2%-23.4%的弓形虫阳性。
结论:这项研究提出了一种新颖的检测方法,可以及时有效地检测针对弓形虫的血清抗体,从而允许其早期临床诊断。此外,使用HMREADER集成数字检测可以增强GICA的实施。
BACKGROUND: Timely diagnosis of Toxoplasma gondii infection is necessary to prevent and control toxoplasmosis transmission. The gold immunochromatographic assay (GICA) is a means of rapidly detecting pathogen in samples. GICA-based diagnostic methods have been developed to accurately detect pathogens with high sensitivity and specificity, and their application in T. gondii diagnosis is expected to yield good results.
METHODS: Colloidal gold test strips were produced using T. gondii C-terminal truncated apical membrane antigen 1 (AMA1C). Colloidal gold-AMA1C and colloidal gold-murine protein conjugate were synthesized under optimal conditions. A nitrocellulose membrane was treated with AMA1C and goat anti-mouse antibody as the test line and control line, respectively. In total, 90 cat serum samples were tested using AMA1C-GICA and a commercial enzyme linked immunosorbent assay (ELISA) kit. The GICA results were digitally displayed using a portable colloidal gold immunochromatographic test strip analyzer (HMREADER). The sensitivity, specificity, and stability of AMA1C-GICA were assessed, and this was then used to examine clinical samples, including 203 human sera, 266 cat sera, and 81 dog sera.
RESULTS: AMA1C-GICA had a detection threshold of 1:32 for T. gondii-positive serum. The GICA strips specifically detected T. gondii antibodies and exhibited no reactivity with Plasmodium vivax, Paragonimus kellicotti, Schistosoma japonicum, Clonorchis sinensis, and Schistosoma mansoni. Consequently, 15 (16.7%) positive samples were detected using the AMA1C-GICA and commercial ELISA kits for each of the assays. The receiver-operating characteristic curve showed that GICA had a relative sensitivity of 85.3% and specificity of 92%, with an area under the curve of 98%. After analyzing clinical samples using HMREADER, 1.2%-23.4% of these samples were found to be positive for T. gondii.
CONCLUSIONS: This study presents a novel assay that enables timely and efficient detection of serum antibodies against T. gondii, thereby allowing for its early clinical diagnosis. Furthermore, the integration of digital detection using HMREADER can enhance the implementation of GICA.