一些microRNAs(miRNA)的表达受到香烟烟雾(CS)的调控,这是主要可预防疾病的主要原因。然而,miRNA的表达是否也受到来自潜在风险降低产物的气溶胶/提取物的调节还没有得到很好的研究.目前的工作是对12项体外研究的荟萃分析,这些研究涉及人类器官型上皮组织的呼吸消化道(口腔,牙龈,支气管,鼻部,和小气道上皮)。这些研究比较了暴露于电子蒸气(电子蒸气)产品和加热烟草产品的气溶胶的影响,以及瑞典鼻烟产品的提取物(在目前的工作中,将被称为降低风险的产物[RRP])对miRNA表达的影响,以及暴露于CS或其总颗粒物分数的影响。该荟萃分析评估了总共736个检测到的miRNA和2775个暴露的培养插入物的12个数据集。t分布随机邻居嵌入方法用于发现以组织类型为特征的miRNA响应的多样性的相似性。曝光类型,和产品浓度。CS诱导的牙龈培养物中miRNA表达的变化与口腔培养物接近;类似地,小气道中miRNA表达的改变,支气管,和鼻组织相似。进行监督聚类以鉴定表现出特定反应模式的miRNA。分析确定了一组miRNA,其表达在暴露于CS后在特定组织中发生了改变(例如,miR-125b-5p,miR-132-3p,miR-99a-5p,和146a-5p)。最后,我们通过在单个miRNA水平上计算RRP和CS诱导的改变之间的反应比r,研究了RRP对miRNA表达相对于CS表达的影响,显示相对于CS暴露,RRP暴露后miRNA表达的改变减少(94%相对减少)。没有特定的miRNA反应模式表明暴露于来自加热的烟草产品和电子蒸汽产品的气溶胶,或者瑞典鼻烟的提取物是可以识别的。
The expression of some microRNAs (miRNA) is modulated in response to cigarette smoke (CS), which is a leading cause of major preventable diseases. However, whether miRNA expression is also modulated by the aerosol/extract from potentially reduced-risk products is not well studied. The present work is a meta-analysis of 12 in vitro studies in human organotypic epithelial cultures of the aerodigestive tract (buccal, gingival, bronchial, nasal, and small airway epithelia). These studies compared the effects of exposure to aerosols from electronic vapor (e-vapor) products and heated tobacco products, and to extracts from Swedish snus products (in the present work, will be referred to as reduced-risk products [RRPs]) on miRNA expression with the effects of exposure to CS or its total particulate matter fraction. This meta-analysis evaluated 12 datasets of a total of 736 detected miRNAs and 2775 exposed culture inserts. The t-distributed stochastic neighbor embedding method was used to find similarities across the diversity of miRNA responses characterized by tissue type, exposure type, and product concentration. The CS-induced changes in miRNA expression in gingival cultures were close to those in buccal cultures; similarly, the alterations in miRNA expression in small airway, bronchial, and nasal tissues resembled each other. A supervised clustering was performed to identify miRNAs exhibiting particular response patterns. The analysis identified a set of miRNAs whose expression was altered in specific tissues upon exposure to CS (e.g., miR-125b-5p, miR-132-3p, miR-99a-5p, and 146a-5p). Finally, we investigated the impact of RRPs on miRNA expression in relation to that of CS by calculating the response ratio r between the RRP- and CS-induced alterations at an individual miRNA level, showing reduced alterations in miRNA expression following RRP exposure relative to CS exposure (94 % relative reduction). No specific miRNA response pattern indicating exposure to aerosols from heated tobacco products and e-vapor products, or extracts from Swedish snus was identifiable.