基于有机染料的生物相容性发光体,具有对溶剂化介质的特性高度敏感的荧光特性,特别感兴趣的是高度敏感,选择性,和易于使用的分析剂。我们发现BODIPY二聚体(2,2\'-,2,3'-3,3'-CH2-bis(BODIPY)(1-3))显示出荧光特性,对极性溶剂的存在具有很高的敏感性。1-3在非极性/低极性溶剂中的强烈荧光在极性介质(丙酮,DMF,和DMSO)。已经确定,CH2-bis(BODIPY)荧光猝灭的主要原因是供电子分子(Solv)对染料的特定溶剂化,并形成稳定的超分子CH2-bis(BODIPY)·2Solv结构。使用稳态吸收和荧光光谱,时间分辨荧光光谱,和计算建模,形成机制,composition,CH2-bis(BODIPY)·2Solv超分子配合物的结构得到证实,并对其稳定性进行了评估。结果表明,有望开发基于CH2-bis(BODIPY)的荧光探针,以检测溶液中有毒的含N/O化合物。
Biocompatible luminophores based on organic dyes, which have fluorescence characteristics that are highly sensitive to the properties of the solvating medium, are of particular interest as highly sensitive, selective, and easy-to-use analytical agents. We found that BODIPY dimers (2,2\'-, 2,3\'-3,3\'-CH2-bis(BODIPY) (1-3)) demonstrate fluorescence characteristics with a high sensitivity to the presence of polar solvents. The intense fluorescence of 1-3 in nonpolar/low-polarity solvents is dramatically quenched in polar media (acetone, DMF, and DMSO). It has been established that the main reason for CH2-bis(BODIPY) fluorescence quenching is the specific solvation of dyes by electron-donating molecules (Solv) with the formation of stable supramolecular CH2-bis(BODIPY)·2Solv structures. Using steady-state absorption and fluorescence spectroscopy, time-resolved fluorescence spectroscopy, and computational modeling, the formation mechanism, composition, and structure of CH2-bis(BODIPY)·2Solv supramolecular complexes have been substantiated, and their stability has been evaluated. The results show the promise of developing fluorescent probes based on CH2-bis(BODIPY)s for detecting toxic N/O-containing compounds in solutions.