目的:喉上神经(SLN)是喉觉的基础,咳嗽反射,和音高控制。SLN损伤具有实质性后果,包括感觉改变,抽吸,和发音障碍.迄今为止,SLN的体内测量仍然难以捉摸。这项研究的目的是评估在大鼠SLN模型中记录运动和感觉诱发电位的可行性。
方法:从4个月大的Sprague-Dawley大鼠中获得了22只大鼠半喉制剂(n=11),并将其纳入本研究。通过在颈动脉附近的内侧延伸点处刺激SLN并在环甲肌上放置记录电极来计算复合运动动作电位(CMAP)和运动单位数估计(MUNE)。通过刺激SLN和喉内收肌反射(LAR)的喉镜可视化来确定感觉反应。对SLN和环甲肌横截面进行染色,并对组织学形态进行定量。
结果:所有试验均成功获得喉诱发电位。平均CMAP潜伏期和阴性持续时间分别为0.99±0.57ms和1.49±0.57ms,分别。MUNE中位数为2.06(IQR1.88,3.51)。以0.69±0.20mV的平均强度诱导LAR。平均轴突数,髓鞘厚度,g比分别为681±192.2、1.72±0.26和0.45±0.04。
结论:本研究证明了记录SLN刺激后诱发反应电位的可行性。我们假设这项工作将提供一个易于处理的动物模型来研究喉感觉和环甲运动功能随着衰老的变化,神经退行性疾病,抽吸,或神经损伤。
方法:N/A喉镜,2023年。
OBJECTIVE: The superior laryngeal nerve (SLN) is fundamental in laryngeal sensation, cough reflex, and pitch control. SLN injury has substantial consequences including altered sensation, aspiration, and dysphonia. To date, in vivo measurement of the SLN remains elusive. The purpose of this study was to assess the feasibility of recording motor and sensory evoked potentials in a rat SLN model.
METHODS: Twenty-two rat hemi-laryngeal preparations (n = 11) were obtained from 4-month-old Sprague-Dawley rats and included in this study. Compound motor action potentials (CMAPs) and motor unit number estimation (MUNE) were calculated by stimulating the SLN at the point of medial extension near the carotid artery and by placing a recording electrode on the cricothyroid muscle. Sensory response was determined through stimulation of the SLN and laryngoscopic visualization of a laryngeal adductor reflex (LAR). SLN and cricothyroid muscle cross-sections were stained and histologic morphometrics were quantified.
RESULTS: Laryngeal evoked potentials were successfully obtained in all trials. Mean CMAP latency and negative durations were 0.99 ± 0.57 ms and 1.49 ± 0.57 ms, respectively. The median MUNE was 2.06 (IQR 1.88, 3.51). LAR was induced with a mean intensity of 0.69 ± 0.20 mV. Mean axon count, myelin thickness, and g-ratio were 681 ± 192.2, 1.72 ± 0.26, and 0.45 ± 0.04, respectively.
CONCLUSIONS: This study demonstrates the feasibility of recording evoked response potentials following SLN stimulation. We hypothesize that this work will provide a tractable animal model to study changes in laryngeal sensation and cricothyroid motor function with aging, neurodegenerative disease, aspiration, or nerve injury.
METHODS: NA Laryngoscope, 134:1778-1784, 2024.