鉴于水和土壤受到Pb(II)的污染,Zn(II),尾矿和酸性矿山排水(AMD)中的其他重金属,我们探讨了硫酸盐还原菌(SRB)对Pb(II)的去除效果,Zn(II),基于微生物处理技术的溶液和尾矿中的其他污染物。我们使用扫描电子显微镜-能量色散光谱(SEM-EDS),X射线衍射(XRD)和X射线荧光(XRF),揭示了SRB处理尾矿的机理。结果表明,SRB在0~40mg/L时对Zn(II)有较强的去除能力;Zn(II)在60~100mg/L时抑制了SRB的生长。同样,SRB表现出非常强的从溶液中去除Pb(II)的能力。在Pb(II)浓度为10-50mg/L时,SRB的去除率为100%。SRB处理可以有效地固定从尾矿中浸出的污染物。随着各层尾矿添加量的增加,SRB处理污染物的能力减弱。当每层添加1厘米的尾砂时,SRB处理尾砂效果最好。治疗后,SO42-的固定化率,Fe(III),Mn(II),Pb(II),Zn(II),Cu(II),#1尾砂渗滤液中总Cr为95.44%,100%,90.88%,100%,96.20%,86.23%,和93.34%,分别。尾矿经SRB处理后,虽然尾矿从松散的颗粒状颗粒凝固成粘性物质,其机械强度<0.2MPa。在通过混合SRB处理尾矿中,脱硫弧菌和脱硫盐藻起着主要作用。尾矿处理过程中混合SRB产生的S2-和碳酸盐可以通过与尾矿释放的重金属离子结合形成FeS来代谢硫酸盐,MnS,ZnS,CuS,PbS,Cr2S3、CaCO3、MnCO3等析出颗粒。这些颗粒附着在尾矿表面,减少尾矿对矿区周围水和土壤的环境污染。
In view of water and soil getting polluted by Pb(II), Zn(II), and other heavy metals in tailings and acid mine drainage (AMD), we explored the removal effect of sulfate-reducing bacteria (SRB) on Pb(II), Zn(II), and other pollutants in solution and tailings based on the microbial treatment technology. We used the scanning electron microscope-energy dispersive spectroscopy (SEM-EDS), X-ray diffraction (XRD), and X-ray fluorescence (XRF), to reveal the mechanism of SRB treatment of tailings. The results showed that SRB had a strong removal capacity for Zn(II) at 0-40 mg/L; however, Zn(II) at 60-100 mg/L inhibited the growth of SRB. Similarly, SRB exhibited a very strong ability to remove Pb(II) from the solution. At a Pb(II) concentration of 10-50 mg/L, its removal percentage by SRB was 100%. SRB treatment could effectively immobilize the pollutants leached from the tailings. With an increase in the amount of tailings added to each layer, the ability of SRB to treat the pollutants diminished. When 1 cm of tailingssand was added to each layer, SRB had the best effect on tailing sand treatment. After treatment, the immobilization rates of SO42-, Fe(III), Mn(II), Pb(II), Zn(II), Cu(II), and total Cr in the leachate of #1 tailing sand were 95.44%, 100%, 90.88%, 100%, 96.20%, 86.23%, and 93.34%, respectively. After the tailings were treated by SRB, although the tailings solidified into a cohesive mass from loose granular particles, their mechanical strength was <0.2 MPa. Desulfovibrio and Desulfohalotomaculum played the predominant roles in treating tailings by mixing SRB. The S2- and carbonate produced by mixing SRB during the treatment of tailings could metabolize sulfate by combining with the heavy metal ions released by the tailings to form FeS, MnS, ZnS, CuS, PbS, Cr2S3, CaCO3, MnCO3, and other precipitated particles. These particles were attached to the surface of the tailings, reducing the environmental pollution of the tailings in the water and soil around the mining area.