在1970年代末,硫烷硫被定义为仅与硫原子共价结合的硫原子。然而,这个定义没有被普遍接受,因为它有点模糊和难以理解。因此,在1990年代初,它被定义为“结合硫,“在用硫醇还原剂还原时容易转化为硫化氢。与H2S相关的结合硫物质包括过硫化物(R-SSH),多硫化物(H2Sn,n≥2或R-S(S)nS-R),和蛋白质结合的元素硫(S0)。目前与H2S相关的许多生物效应可归因于过硫化物和多硫化物。在20世纪,“硫烷硫”的定量测定通常使用称为氰解的反应进行。在过去的30年中已经开发了几种方法。目前用于检测H2S和多硫化物的方法包括亚甲蓝形成的比色测定法,硫离子选择性或极谱电极,火焰光度或硫化学发光检测的气相色谱,硫化物荧光衍生高效液相色谱分析,液相色谱与串联质谱,生物素开关技术,以及硫化物或多硫化物敏感荧光探针的使用。在这次审查中,我们讨论了迄今为止报道的测量硫烷硫的方法和使用这些方法获得的结果。
In the late 1970s, sulfane sulfur was defined as sulfur atoms covalently bound only to sulfur atoms. However, this definition was not generally accepted, as it was slightly vague and difficult to comprehend. Thus, in the early 1990s, it was defined as \"bound sulfur,\" which easily converts to hydrogen sulfide upon reduction with a thiol-reducing agent. H2S-related bound sulfur species include persulfides (R-SSH), polysulfides (H2Sn, n ≥ 2 or R-S(S)nS-R, n ≥ 1), and protein-bound elemental sulfur (S0). Many of the biological effects currently associated with H2S may be attributed to persulfides and polysulfides. In the 20th century, quantitative determination of \"sulfane sulfur\" was conventionally performed using a reaction called cyanolysis. Several methods have been developed over the past 30 years. Current methods used for the detection of H2S and polysulfides include colorimetric assays for methylene blue formation, sulfide ion-selective or polarographic electrodes, gas chromatography with flame photometric or sulfur chemiluminescence detection, high-performance liquid chromatography analysis with fluorescent derivatization of sulfides, liquid chromatography with tandem mass spectrometry, the biotin switch technique, and the use of sulfide or polysulfide-sensitive fluorescent probes. In this review, we discuss the methods reported to date for measuring sulfane sulfur and the results obtained using these methods.