未经证实:1型糖尿病的特征是血糖水平升高,对整个身体的多个器官和组织产生负面影响,其患病率正在上升。先前的报告主要调查了糖尿病患者的血清和尿液样本。然而,只有少数研究检查了糖尿病动物或患者的整体代谢状况。目前的系统调查将有助于了解基于STZ的1型糖尿病发病机制。
未授权:将雄性SD大鼠任意地分成对照和链脲佐菌素(STZ)处理的糖尿病大鼠(n=7)。实验大鼠每天腹腔注射50mg/kgSTZ,连续2天。6周后,代谢物通过气相色谱-质谱(GC-MS)进行评估,多变量分析用于筛选诱导的糖尿病大鼠和正常大鼠之间的差异表达(DE)代谢物。
未经鉴定:我们鉴定了血清中的18、30、6、24、34、27、27和12种DE代谢物,心,肝脏,肾,皮质,肾脂,海马体,和STZ处理的糖尿病大鼠的棕色脂肪组织,与对照大鼠相比。根据我们的分析,在氨基酸(AAs)中观察到最大的差异,B族维生素,和嘌呤配置文件。使用代谢途径分析,我们筛选了与STZ暴露的糖尿病发病机制相关的13条代谢途径.这些途径主要是AA代谢,其次是有机酸,糖,糖和脂质代谢。
未经评估:根据我们的GC-MS分析,我们确定了暴露于STZ的糖尿病大鼠体内潜在的代谢改变,这可能有助于理解糖尿病的发病机制。
Type 1 diabetes is characterized by elevated blood glucose levels, which negatively impacts multiple organs and tissues throughout the body, and its prevalence is on the rise. Prior reports primarily investigated the serum and urine specimen from diabetic patients. However, only a few studies examined the overall metabolic profile of diabetic animals or patients. The current systemic investigation will benefit the knowledge of STZ-based type 1 diabetes pathogenesis.
Male SD rats were arbitrarily separated into control and streptozotocin (STZ)-treated diabetic rats (n = 7). The experimental rats received 50mg/kg STZ intraperitoneal injection daily for 2 consecutive days. Following 6 weeks, metabolites were assessed via gas chromatography-mass spectrometry (GC-MS), and multivariate analysis was employed to screen for differentially expressed (DE) metabolites between the induced diabetic and normal rats.
We identified 18, 30, 6, 24, 34, 27, 27 and 12 DE metabolites in the serum, heart, liver, kidney, cortex, renal lipid, hippocampus, and brown fat tissues of STZ-treated diabetic rats, compared to control rats. Based on our analysis, the largest differences were observed in the amino acids (AAs), B-group vitamin, and purine profiles. Using the metabolic pathway analysis, we screened 13 metabolic pathways related to the STZ-exposed diabetes pathogenesis. These pathways were primarily AA metabolism, followed by organic acids, sugars, and lipid metabolism.
Based on our GC-MS analysis, we identified potential metabolic alterations within the STZ-exposed diabetic rats, which may aid in the understanding of diabetes pathogenesis.