由于各种原因,昆虫中的基于光的事件值得越来越多的关注。除了它们在特定间和特定内视觉传达中的作用外,生物,生态和分类学意义,光学特性也是监测害虫和疾病媒介的有前途的工具。其中包括亚洲虎蚊,白纹伊蚊,全球虫媒病毒载体.在这里,我们专注于Ae的自发荧光表征。使用联合成像和荧光光谱方法的白纹西亚成年人。成像已经证明,自发荧光从特定的身体区室上升,比如头部附属物,腹部和腿部的鳞片。荧光光谱法表明,发射由410-600nm区域的主带组成。最大峰值位置的变化,在430nm和500nm之间,在光谱宽度上,取决于目标结构,表明存在,在不同的程度,不同的荧光团,很可能是reslin,几丁质和黑色素.这项工作的目的是为迄今为止尚未开发的Ae自发荧光提供初步证据。白纹,为建立特定物种和性别的生物功能调查以及飞行中直接检测和监测蚊子媒介的策略提供新的视角。
Light-based events in insects deserve increasing attention for various reasons. Besides their roles in inter- and intra-specific visual communication, with biological, ecological and taxonomical implications, optical properties are also promising tools for the monitoring of insect pests and disease vectors. Among these is the Asian tiger mosquito, Aedes albopictus, a global arbovirus vector. Here we have focused on the autofluorescence characterization of Ae. albopictus adults using a combined imaging and spectrofluorometric approach. Imaging has evidenced that autofluorescence rises from specific body compartments, such as the head appendages, and the abdominal and leg scales.
Spectrofluorometry has demonstrated that emission consists of a main band in the 410-600 nm region. The changes in the maximum peak position, between 430 nm and 500 nm, and in the spectral width, dependent on the target structure, indicate the presence, at variable degrees, of different fluorophores, likely resilin, chitin and melanins. The aim of this work has been to provide initial evidence on the so far largely unexplored autofluorescence of Ae. albopictus, to furnish new perspectives for the set-up of species- and sex-specific investigation of biological functions as well as of strategies for in-flight direct detection and surveillance of mosquito vectors.